Homogeneous time-resolved

Homogeneous Time Resolved Fluorescence (HTRF) (Cisbio International) is an assay based on the proximity of a lanthanide cryptate donor and a fluorescent acceptor molecule whose excitation wavelength overlaps that of the cryptate s emission. The utility of this technique is based on the time resolved fluorescence properties of lanthanides. Lanthanides are unique in the increased lifetime of their fluorescence decay relative to other atoms, so a delay in collection of the emission intensity removes the background from other fluorescent molecules. An example of the HTRF assay is a generic protein-protein interaction assay shown in Fig. 2. 

Cencic, R., Yan, Y., and Pelletier, J. (2007). Homogenous time resolved fluorescence assay to identify modulators of cap-dependent translation initiation. Comb. Chem. High Throughput Screen 10, 181-188. 

Blomberg, K., Hurskainen, P. and Hemmila, I. (1999). Terbium and rhodamine as labels in a homogeneous time-resolved fluorometric energy transfer assay of the b-subunit of human chorionic gonadotropin in serum. Clin. Chem. 45, 855-61. 

Maurel, D., Kniazeff, J., Mathis, G., Trinquet, E., Pin, J. P. and Ansanay, FI. (2004). Cell surface detection of membrane protein interaction with homogeneous time-resolved fluorescence resonance energy transfer technology. Anal. Biochem. 329, 253-62. 

Liang, A.M., Claret, E., Ouled-Diaf, J., Jean, A., Vogel, D., Light, D.R., Jones, S.W., Guilford, W.J., Parkinson, J.F., and Snider, R.M. (2007) Development of a homogeneous time-resolved fluorescence leukotriene B4 assay for determining the activity of Leukotriene A4 hydrolase. J. Biomol. Screen. 12, 536-545. 

L Hemmila, O. Malminen, H. Mikola, and T. Lovgren, Homogeneous time-resolved fluoroimmu-noassay of thyroxin in serum, Clin. Chem. 34, 2320-2322 (1988). 

HTRF (homogeneous time-resolved fluorescence) LANCE (lanthanide chelate excitation) Cisbio International PerkinElmer Life Sciences Lanthanide TR-FRET using Eu3+ cryptate/chelate-donor fluorophore and cross-linked aUophycocyanin-acceptor fluorophore... 

Kolb, A.J., Burke, J.W., and Mathis, G., Homogeneous, time-resolved fluorescence method for drug discovery, in High Throughput Screening, The Discovery of Bioactive Substances, Devlin, J.P., Ed., Marcel Dekker, New York, 1997, chap. 19. 

Wang, G., J. Yuan, K. Matsumoto, et al. 2001. Homogeneous time-resolved fluoroimmunoassay of bensulfuron-methyl by using terbium fluorescence energy transfer. Talanta 55 1119-1125. 

Fig. 13. Structure of TBP-Eu3+ and principle of TRACE homogeneous time-resolved fluoroimmunoassays.

Ferrer, M. et al. 2003 Miniaturizable homogeneous time-resolved fluorescence assay for carboxypeptidase B activity. Anal. Biochem. 317. 94-98. 

Karvinen, J. et al. 2002. Homogeneous time-resolved fluorescence quenching assay (LANCE) for caspase 3. J. Biomol. Screen. 1, 223-231. 

Primary Homogeneous Time-Resolved Fluorescence (HTRF) HTS Assay.84... 

PRIMARY HOMOGENEOUS TIME-RESOLVED FLUORESCENCE (HTRF) HTS ASSAY... 

Inherent fluorescence in natural product samples rules out the use of frank fluorescence as an assay readout. In the early 1990s, the development of homogeneous time-resolved fluorescence (HTRF) allowed the use of fluorescence with such samples (Kolb, Yamanaka, and Manly, 1996 Kolb and Manly, 1997 Bazin, Trinquet, and Mathis, 2002 Mathis, 1999). This technique... 

Because of the very stringent reliability requirements for diagnostic applications, the only homogeneous techniques that have survived in this field are the fluorescence polarization (FP) methods originally developed by AbbotE and the homogeneous time resolved fluorescence (HTRF) and TRACE approaches developed later by CIS bio international. 

Hemmila, L, Malminen, O., Mikola, H., and Lovgren, T. (1988). Homogeneous Time-Resolved Fluoroimmunoassay of Thyroxine in Serum. Clin. Chem. 34 2320-2322. 

The homogeneous TRF assay formats HTRF ( homogeneous time-resolved fluorescence ) by GisBio International [115, 120, 136, 137] and LANGE ( lantha-... 

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