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High-throughput technique with

Discriminating on one hand the bead type and on the other hand the hybridized sequence leads to a sensitive and high throughput technique with detection limits in the lOfmole range [24]. Finally, as a prospective issue, flow cytometry is planned to have a potential throughput of nearly 300 thousand analyses per day, and to play an important role in genomics and proteomics [25]. [Pg.121]

Figure 6.1 Basic concept of the application of FTIR imaging as a high-throughput technique. Placing multiple samples in the field of view of the imaging spectrometer allows the collection of a single dataset that contains information from all samples. Reprinted with permission from ACS. Figure 6.1 Basic concept of the application of FTIR imaging as a high-throughput technique. Placing multiple samples in the field of view of the imaging spectrometer allows the collection of a single dataset that contains information from all samples. Reprinted with permission from ACS.
The choice of using low-, medium- or high-throughput techniques depends on the particular question being dealt with and the required outcome of the study. Each of these three levels is considered individually in this section, although the boundaries among the three levels cannot be simply defined. [Pg.258]

Plastic microdevices for high-throughput screening with MS detection were also prepared for detection of aflatoxins and barbiturates. These devices incorporated concentration techniques interfaced with electrospray ionization MS (ESI-MS) through capillaries [2], The microfluidic device for aflatoxin detection employed an affinity dialysis technique, in which a poly (vinylidene fluoride) (PVDF) membrane was incorporated in the microchip between two channels. Small molecules were dialyzed from the aflatoxin/antibody complexes, which were then analyzed by MS. A similar device was used for concentrating barbiturate/antibody complexes using an affinity ultrafiltration technique. A barbiturate solution was mixed with antibodies and then flowed into the device, where uncomplexed barbiturates were removed by filtration. The antibody complex was then dissociated and electrokinetically mobilized for MS analysis. In each case, the affinity preconcentration improved the sensitivity by at least one to two orders of magnitude over previously reported detection limits. [Pg.429]


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High throughput technique

High-throughput

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