Sequencing by hybridization

SoLidiern, E. M., 1996. DNA chips Analysing sequence by hybridization to oligoiiLicleoddes on a large scale. Trends in Genetics 12 110-115. 

Probe Single-stranded DNA or RNA molecules of specific base sequence, labeled either radioactively or immunologically, that are used to detect the complementary base sequence by hybridization. 

However, if the gene has been cloned, but the required activity is not produced, then the functional test will fail to pick up the target gene. In this case, if some gene sequence information is available, then it may be possible to test for the presence of DNA with the expected sequence by hybridization with radio-labelled probe DNA or, more usually, by PCR. This sequence-based screening test could pick up positives which have been missed in the initial screen because the gene has been successfully cloned but the enzyme has not been produced in an active form (perhaps because expression has not occurred or because E. coli is a poor host to support production of active enzyme), or where there is no convenient function-based assay available. 

Drmanac, S. and Drmanac, R., Processing of cDNA and genomic kilobase-size clones for massive screening, mapping and sequencing by hybridization. Biotechniques, 17, 328-336, 1994. 

Southern, E.M., Maskos, U., and Elder, J.K., Analyzing and comparing nucleic acid sequences by hybridization to arrays of oligonucleotides evaluation using experimental models, Gmomics, 13, 1008-1017, 1992. 

Microarray Methods. Affymetrix and Hyseq Resequencing AND Sequencing by Hybridization... 

Wallace RB, Shaffer J, Murphy RE, Bonner J, Hirose T, Itakura K (1979) Nucleic Acids Research 6 3542-3557 Fodor SPA, Lipshutz RJ, Huang X (1993) DNA sequencing by hybridization, Proceedings of the Robert A. Welch Foundation 37th Conference on Chemical Research - 40 Years of the DNA Double Helix. Robert A. Welch Foundation, Houston,p 3-9... 

Homogeneous hybridization assays are analyses for the detection of a given DNA/RNA sequence by hybridization without separation processes (B/F separation). These assays form a basis of real-time PCR as described below. Furthermore, sufficiently sensitive homogeneous hybridization assays could be done without PCR and enable, for example, direct detections of certain mRNA forms expressed in cells. This type of experiment has been actually performed with some organic fluorophores (Peng et al., 2005 Santangelo et al., 2006). 

Southern, E.M. (1996) DNA Chips Analyzing Sequence by Hybridization to Oligonucleotides on a Large Scale, Trends Genet. 12,110-115. 

Strezoska, Z., Paunesku, T., Radosavljevic, D., Labat, I., Drmanac, R., and Crkven-jakov, R. (1991). DNA sequencing by hybridization 100 bases read by a non-gel-based method. Proc. Natl. Acad. Sci. USA 88,10089 10093. 

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