High throughput technique sample preparation

Single-enzyme systems, typically obtained from cDNA expression systems, are an effective technique for the study of individual enzyme reactions and their products. The incubation of enzyme, buffer, necessary cofactors, and drug produces extremely clean samples that are amenable to either simple cleanup procedures or direct injection into the LC/MS instrument (Chap. 6). These isolated enzymes can be stored at -80°C for extended periods of time, making them readily available whenever necessary. In addition, they are reasonably easy to work with either by hand or in a robotic environment where high-throughput incubation, sample preparation, and analysis are possible. 

Cell migration was assayed by automated time-lapse video microscopy. In this technique, cells are imaged on the surfaces and the individual cell tracks are reconstructed from the position of each cell at successive time points. The automation enables time lapse videos to be taken from multiple locations on the same surface at the same time. Thus, the entire gradient can be imaged at once. This high-throughput technique combined with the combinatorial sample preparation enables the exploration of a relatively large parameter space quickly. 

I High-Throughput Sample Preparation Techniques and Their Application to Bioanalytical Protocols and Purification of Combinatorial Libraries... 

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