High-pressure liquid chromatography resolution

The stationary phase matrices used in classic column chromatography are spongy materials whose compress-ibihty hmits flow of the mobile phase. High-pressure liquid chromatography (HPLC) employs incompressible silica or alumina microbeads as the stationary phase and pressures of up to a few thousand psi. Incompressible matrices permit both high flow rates and enhanced resolution. HPLC can resolve complex mixtures of Upids or peptides whose properties differ only slightly. Reversed-phase HPLC exploits a hydrophobic stationary phase of... 

Abel, C.B.L., Lindon, J.C., Noble, D., Rudd, B.A.M., Sidebottom, P.J., and Nicholson, J.K, Characterization of metabolites in intact Streptomyces citricolor culture supernatants using high-resolution nuclear magnetic resonance and directly coupled high-pressure liquid chromatography-nuclear magnetic resonance spectroscopy, Anal. Biochem., 270, 220, 1999. 

GC = gas chromatography, Py = pyrolysis (flash or hydrous heating), MS = mass spectrometry (HR = high resolution), IRMS = isotope ratio mass spectrometry, HPLC = high-pressure liquid chromatography, EC = electron capture. Cl = chemical ionization, NMR = nuclear magnetic resonance spectrometry. X-ray = X-ray crystallography. 

High Pressure Liquid Chromatography. In plasma UV detection, comparison with a GC method—R. N. Gupta and F. Eng, J. High Resolut. Chromat. Chromat. Commun., 1980, i, 419-420. 

High Pressure Liquid Chromatography. In serum canrenone, sensitivity 5 ng/ml, UV detection—F. Besenfelder and R. Endele, J. High Resolut. Chromat., Chromat. Commun., 1981, 4, 419-421. In serum or urine canrenone, detection limit 5 ng/ml in serum, UV detection—G. B. Neurath and D. Ambrosius, J. Chromat., 1979,163, Biomed. Appl., 5, 230-235. 

High-Pressure Liquid Chromatography. There appears to be little doubt that HPLC will play a prominent part in future separation of shale oil components. Liquid-phase methods have long been important in work-up procedures for analysis and their extension to high resolution areas is under extensive study. One particular example is chosen to illustrate the capabilities of HPLC that of its combination with reactive pre-columns for quantitative estimation of oil compound types. 

A few years later, by using the same experimental procedures, the same scientists prepared cyclic polybutadienes170 (Scheme 82). THF was the polymerization solvent, which results in a 60% 1,2-content. From the g values was concluded that some of the cyclic polybutadienes (PBd) were contamined by their linear precursors. A high-resolution column set was used in order to separate the linear from the cyclic polymer. In this work the characterization analysis was the most comprehensive presented so far. Recently, these cyclic PSs were analyzed by high-pressure liquid chromatography under critical conditions, which is a method that can separate linear and cyclic macromolecules according to their archi-... 

DNP = dinitrophenol ELCD = electrochemical detection FID = flame ionization detection GC = gas chromatography HPLC = high pressure liquid chromatography HRGC = high resolution gas chromatography MS = mass spectrometry PAD = pulse amphrometric detection PID = photodiode array detection UV = ultraviolet detection... 

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