High pressure liquid chromatography HPLC , proteins

G Seipke, H Mtillner, U Grau. High-pressure liquid chromatography (HPLC) of proteins. Angew Chem Int Ed Engl 25 535-552, 1986. 

Heme proteins 373 Heparin 803, 819, e9 binding site 805 Hepatitis C virus 381, 384, 391 Herbal extracts e277, e279 Heterogeneous catalysis 932 immunoassays 924 Hexadecanethiol 243 Hg 521, 523-524, 526 Hg-Au amalgam 236 High-pressure liquid chromatography (HPLC) 212... 

The determination (3) of physical and chemical properties such as solubility, stability, pKa, glassbinding and protein-binding of A -tetrahydrocannabinol 1 and correlated congeners were only possible after the development of new high pressure liquid chromatography (HPLC) techniques and the modification of gas liquid chromatography (GLC) technologies. 

Figure 3.6 High-pressure liquid chromatography (HPLC). Gel filtration by HPLC clearly defines the individual proteins because of its greater resolving power (1) thyroglobulin (669 kd), (2) catalase (232 kd), (3) bovine serum albumin (67 kd), (4) ovalbumin (43 kd), and (5) ribonuclease (13.4 kd). [After K. J. Wilson and T, D. Schlabach. In Current Protocols in Molecular Biology, vol. 2, suppl. 41, F. M. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl. Eds. (Wiley, 1998), p, 10.14.1.]...

FIGURE 4.6 High-pressure liquid chromatography (HPLC). Gel filtration by HPLC clearly defines the individual proteins because of its greater resolving power (1) thyroglobulin (669 kd),... 

After reaction with the labeled reagents, the protein is treated with proteolytic enzymes thermolysin, chymotrypsin, and trypsin have been used for proteolysis of the labeled macromolecule. The resulting peptides are separated by high-pressure liquid chromatography (HPLC), and the amount of radioactivity of each eluted peak is measured. Then the labeled peptides are analyzed after acid hydrolysis and the amount of radioactive label incorporated into individual amino acid side chains is measured. 

Isolation of individual amino acids started about 1820 by 1904 all of the naturally occurring amino acids in proteins had been isolated except methionine (Mueller, 1922) and threonine (Rose, 1937). One of the earliest methods for the separation of amino acids was through the differential volatility of their methyl or ethyl esters (Emil Fischer, 1901). This approach led to the discovery of valine, proline, and hydroxyproline. [In the 1970s Fischer s method was modified for microanalysis of proteins, separating the amino acid esters by gas phase chromatography. Separation is now usually performed by hplc (high pressure liquid chromatography).]... 

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