High-performance size exclusion refractive index detectors

High Performance Size Exclusion Chromatography. The Hewlett-Packard 1090 liquid chromatograph was used with the HP 1040 diode array or HP 1037A refractive index (and HP 3392 integrator) detectors. A fifty A (5 mm, 300 x 7 mm) Polymer Laboratories PL gel (polystyrene-divinylbenzene copolymer gel) column was used and standards were as described in Chum et al. (13). Tetrahydrofuran solutions of oil and oil fractions were analyzed. [Pg.142]

The determination of the molecular weight of nanoparticles is performed by gel permeation chromatography (GPC). The experimental setup consists of a high performance liquid chromatography system with a size exclusion column and a refractive index detector. The nanoparticles are usually freeze-dried and dissolved in tetrahydrofuran for analysis on the system. Poly(styrene) or poly(methylmethacrylate) standards are used to calibrate the column, to enable the determination of number average molecular weight (Mn), as in... [Pg.10]