High-performance liquid chromatography mycotoxins

Vesonder, R.F., Peterson, R.E., and Weisleder, D. 1990. Fumonisin By. Isolation from com culture, and purification by high performance liquid chromatography. Mycotoxin Res. 6 85-88. 

Chemical analysis of hair samples may also provide a method for examining chronic mycotoxin exposures. In 2003, Sewram et al. (2003) reported that human hair testing could be used to detect fumonisins. After extraction and clean up, high performance liquid chromatography coupled to electrospray ionization-mass spectrometry (HPLC-ESI-MS) was able to detect fumonisin Bi, fumonisin B2, and fumonisin B3 from human hair samples (Sewram et al., 2003). However, these were... 

Jimenez, M. and Mateo, R. 1997. Determination of mycotoxins produced by Fusarium isolates from banana fruits by capillary gas chromatography and high-performance liquid chromatography. 

The ELISA tests are preferred because they require low volume of the sample and fewer clean-up procedures of the extracted sample compared to the conventional methods like TLC (thin layer chromatography) and HPLC (high-performance liquid chromatography). ELISA tests are rapid, simple, specific, sensitive and portable for use in the field for the detection of mycotoxins in foods and fodder (Zheng et al. 2005). 

High-performance liquid chromatography (HPLC) remains the most popular method for determination of ochratoxin A. Most HPLC methods use a reversed-phase column and an acidic mobile phase, so the carboxyl group of the mycotoxin is in the undissociated form. The post-column addition of 10% ammonia solution increases the fluorescence emission of ochratoxin A by a factor of 10 (Monaci Palmisano, 2004). 

Oueslati, S., Romero-Gonzalez, R., Lasram, S., Frenich, A. G., and Vidal, J. M. 2012. Multi-mycotoxin determination in cereals and derived products marketed in Tunisia using ultra-high performance liquid chromatography coupled to triple quadrupole mass spectrometry. Food Chem. Toxicol. 50 2376-2381. 

Since NIV occurs as a co-contaminant with other trichothecene mycotoxins, it is often analyzed simultaneously with the co-contaminants rather than alone. Analytical methods developed so far include thin layer chromatography (TLC) capillary gas chromatography (GC) with electron-capture detection (BCD), flame ionization detection (FID), or mass spectrometric detection (GC/MS) high-performance liquid chromatography (HPLC) with ultraviolet (UV), fluorescence, or mass spectrometric detection supercritical fluid chromatography (SFC) and time-of-flight mass spectrometry (LC/TOF-MS). 

Martos PA, Thompson W, Diaz GJ (2010) Multiresidue mycotoxin analysis in wheat, barley, oats, rye and maize grain by high-performance liquid chromatography-tandem mass spectrometry. World Mycotox J 3 205-223... 

Fumonisins are recently discovered mycotoxins which cause leukoencephalomalacia in horses and pulmonary edema in swine (9). The toxicity of the fumonisins is currently being assessed. The fumonisins are analyzed by high-performance liquid chromatography (HPLC) or TLC. 

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