High-performance liquid chromatography injection valve

High Performance Liquid Chromatography. All separations were performed using an Altex Scientific (1780 Fourth Street, Berkeley, CA 94710) Model 320 Advance Research Chromatograph, consisting of a model lOOA dual piston analytical pump, a Model 153 UV detector, a Model 210 injection valve, and a Model 155 recorder. The columns (600 x7.5mm) evaluated were the Spherogel TSK-SW-2000 and SW-3000 (Altex). Unless otherwise stated, all separations were carried out at 23 - 25 . 

Caprioli and Lin first described this method for the characterization of penicillin G in 1990 [19]. A diagram of the microdialysis/cfFAB experimental setup used is shown in Fig. 8. Dialysate was allowed to collect in a 20- dL sampling loop via syringe pump. After collection, the injection valve was switched and a high-performance liquid chromatography pump was used to deliver the microdialysis sample to the mass spectrometer. Mass spectra of penicillin from both a standard and an in vivo microdialysis sample (shown in Fig. 9) compare well. 

Figure 11.6 Schematic diagrams of a typical injection valve used for high performance liquid chromatography (a) load position (b) inject position.

Asafu-Adjaye, E.B. Su, S.Y. Shiu, G.K. Switching-valve-filter technique for the direct injection and analysis of drugs in plasma using high-performance liquid chromatography. J.Chromatogr.B, 1994, 652, 35-42... 

FIGURE 24.6 FIA manifold for online dialysis coupled with HPLC. AS acceptor stream C carrier DC dialysis cell DM dialysis membrane DS donor stream HPLC high-performance liquid chromatography IV injection valve MCI and MC2 mixing coils PI and P2 pumps S sample W waste. 

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