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High performance liquid chromatography HPLC principle

The principle was demonstrated using triazine herbicides as templates and by varying the type of functional monomer and the monomer composition. With a final batch size of ca. 40 mg of monomer, the consumption of monomers and template is significantly reduced and the synthesis and evaluation can take place in standard high-performance liquid chromatography (HPLC) autosample vials. After synthesis,... [Pg.188]

High-performance liquid chromatography (HPLC) and fast protein liquid chromatography (FPLC) rely on the same separation principles as the traditional chromatography columns, but tend to be much faster because of high flow rates that are possible due to the uniform bead size and the mechanical strength of the beads. See also Chapter 4, section 1.2.2. [Pg.66]

Application and Principle This procedure is used to determine maltogenic amylase activity in preparations derived from Bacillus subtilis containing a Bacillus stearothermophilus amylase gene. The test is based on a 30-min hydrolysis of malto-triose under controlled conditions and measurement of the glucose formed by high-performance liquid chromatography (HPLC). [Pg.916]

This chapter introduces some of the basic concepts and principles of liquid chromatography, providing background on the development of high performance liquid chromatography (HPLC) and briefly describing the basic system components. [Pg.13]

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