High-performance liquid chromatography computers

Adaptation of the modified factorial techniques to desktop computers has also been accomplished [24, 25]. Down et al. [25] presented this concept and applied the programs to a tablet problem. The statistics involved were presented in some detail. A similar design was also used to study a high-performance liquid chromatography (HPLC) analysis [26]. In an unusual application, optimization techniques were even used to study the formulation of a culture medium in the field of virology [27]. 

Glajch, J.L., Snyder, L.R., editors (1990). Computer-Assisted Method Development for High-Performance Liquid Chromatography. Elsevier, Amsterdam. 

L. van Heukelem and C.S. Thomas, Computer assisted high-performance liquid chromatography method development with applications to the isolation and analysis of phytoplankton pigments. J. Chromatogr.A 910 (2001) 31—49. 

D Commercial COTS controlled by external computer Hybrid systems such as automated dissolution workstation with high-performance liquid chromatography (HPLC) or ultraviolet-visible (UV-Vis) interface Liquid chromatographs, gas chromatographs, UV/Vis spectrophotometers, Fourier transform infrared (FTIR) spectrophotometers, near-infrared (NIR) spectrophotometers, mass spectrometers, atomic absorption spectrometers, thermal gravimetric analyzers, COTS automation workstations... 

Free energies of activation for the enantiomerization of a series of iV-aryl-1,3,2-benzodithiazole 1-oxides 41 have been determined by dynamic high-performance liquid chromatography (DHPLC) on a chiral stationary phase <1999JOC1483>. From a comparison of experimental and computer-simulated chromatograms, the barriers to stereoinversion at sulfur were found to be around 80 kj mol 1 and relatively insensitive to effects from substituents in the iV-aryl group. 

A. F. Fell, H. P. Scott, R. Gill, and A. C. Moffat, Novel techniques for peak recognition and deconvolution by computer aided photodiode array detection in high-performance liquid chromatography, J. Chromatogr., 282 123 (1983). 

Mass spectrometry (MS) is now a well-accepted tool for the identification as well as quantitation of unknown compounds. The combination of MS with powerful separation methods such as gas chromatography (GC) or high-performance liquid chromatography (LC) provides a technique which is widely accepted for the identification of unknown components in complex mixtures from a wide variety of problems such as environmental pollutants, biological fluids, insect pheromones, chemotaxonomy, and synthetic fuels. The importance of such analyses has grown exponentially in the last few years there are now well over a thousand GC/MS instruments in use around the world, most with dedicated computer systems which make possible the collection from each of hundreds of unknown mass spectra per day (1). 

T. H. Hoang, D. Cuerrier, S. McChntock, and M. Di Maso, Computer-assisted method development and optimization in high-performance liquid chromatography,/. Chromatogr. A 991 (2003), 281-287. 

High-performance liquid chromatography (HPLC) provides an excellent platform to carry out these measurements. The computer-controlled HPLC with automated sample handling, injection and computerised data acquisition and processing makes possible the measurement of hundreds of samples per week without too much manual work. The measurements are made by running overnight and at weekends so this requires... 

The experiments within the frame of the REHE project were performed in the aqueous phase in a discontinuous, batch-wise manner. It was necessary, in order to get a statistically significant result, to repeat the same experiment several hundred or even several thousand times with a cycle time of typically 45 s. These studies were performed with the Automatic Rapid Chemistry Apparatus (ARCA) II (SchSdel et al, 1989), a computer-controlled apparatus for fast, repetitive high-performance liquid chromatography (HPLC) separations. A schematic of the ARCA II components is shown in Figure 6.2. 

Column and high performance liquid chromatography (HPLC) methods for measurement of solubility, octanol-water partition coefficient, and vapor pressure which are replacing the older equilibrium methods tend to underestimate aqueous solubility and vapor pressure and tend to overestimate the octanol-water partition coefficient. The standard deviation for both the equilibrium and dynamic systems are similar, but calibration between systems is necessary to insure that they agree. The range of errors for both types of measurement as mentioned in the literature are well within the range predicted by the computer-simulated error distributions generated in this report. The measurement error... 

---