Chiral separation high-performance liquid chromatography

Sharp, V.S. et al.. Enantiomeric separation of dansyl amino acids using macrocyclic antibiotics as chiral mobile phase additives by narrow-bore high-performance liquid chromatography. Chirality, 16, 153, 2004. 

The need for chiral high-performance liquid chromatography (HPLC) separation to upgrade the ee. 

Caccamese, S. Direct high-performance liquid chromatography (HPLC) separation of etodolac enantiomers using chiral stationary phases. Chirality, 1993, 5, 164-167... 

Tan X, Hou S, Wang M (2007) Enantioselective and diastereoselective separation of synthetic pyrethroid insecticides on a novel chiral stationary phase by high-performance liquid chromatography. Chirality 19 574—580... 

Despite the difficulties caused by the rapidly expanding literature, the use of chiral stationary phases (CSPs) as the method of choice for analysis or preparation of enantiomers is today well established and has become almost routine. It results from the development of chiral chromatographic methods that more than 1000 chiral stationary phases exemplified by several thousands of enantiomer separations have been described for high-performance liquid chromatography (HPLC). 

Capillary electrophoresis employing chiral selectors has been shown to be a useful analytical method to separate enantiomers. Conventionally, instrumental chiral separations have been achieved by gas chromatography and by high performance liquid chromatography.127 In recent years, there has been considerable activity in the separation and characterization of racemic pharmaceuticals by high performance capillary electrophoresis, with particular interest paid to using this technique in modem pharmaceutical analytical laboratories.128 130 The most frequently used chiral selectors in CE are cyclodextrins, crown ethers, chiral surfactants, bile acids, and protein-filled... 

Mangani, F., Luck, G., Fraudeau, C., Verette, E. (1997). Online column-switching high-performance liquid chromatography analysis of cardiovascular drugs in serum with automated sample clean-up and zone-cutting technique to perform chiral separation. J. Chromatogr. A 762, 235-241. 

Enantiomers of the 8,9-dichloro-2,3,4,4 ,5,6-hexahydro-177-pyrazino[l,2-tf]quinoxalin-5-one (structure 249 Rz = R3 = Cl R1 = R4 = H) could be separated by normal-phase, chiral high-performance liquid chromatography (HPLC) with increased retention and separation factors if ethoxynonafluorobutane was used as solvent, instead of -hexane <2001JCH(918)293>. 

The above chemistry has been applied to the synthesis of a series of derivatives which show activity against animal parasites. In order to confirm further the stmcture and configuration of the most active enantiomer of one of these compounds, the enantiomers were separated by chiral high-performance liquid chromatography (HPLC), and single crystal X-ray diffraction of a 2 1 CuCl2 complex was carried out <2005BML2375>. 

Aboul-Enein and Ali [78] compared the chiral resolution of miconazole and two other azole compounds by high performance liquid chromatography using normal-phase amylose chiral stationary phases. The resolution of the enantiomers of ( )-econazole, ( )-miconazole, and (i)-sulconazole was achieved on different normal-phase chiral amylose columns, Chiralpak AD, AS, and AR. The mobile phase used was hexane-isopropanol-diethylamine (400 99 1). The flow rates of the mobile phase used were 0.50 and 1 mL/min. The separation factor (a) values for the resolved enantiomers of econazole, miconazole, and sulconazole in the chiral phases were in the range 1.63-1.04 the resolution factors Rs values varied from 5.68 to 0.32. 

Ye J, Wu J, Liu W (2009) Enantioselective separation and analysis of chiral pesticides by high-performance liquid chromatography. Trends Anal Chem 28 1148-1163... 

Ding, GS. etal.. Chiral separation of enantiomers of amino acid derivatives by high-performance liquid chromatography on a norvancomycin-bonded chiral stationary phase, Talanta, 62, 997, 2004. 

Bosakova, Z., Cufinovd, E., and Tes ovd, E., Comparison of vancomycin-based stationary phases with different chiral selector coverage for enantioselective separation of selected drugs in high-performance liquid chromatography, J. Chromatogr. A, 1088, 94, 2005. 

Torok, G. et al.. Direct chiral separation of unnatural amino acids by high performance liquid chromatography on a ristocetin A-bonded stationary phase. Chirality, 13, 648, 2001. 

Achiral (separation of diastereomeric derivatives) and chiral (sepa-ration of enantiomers) chromatography gas chromatography high-performance liquid chromatography super- and sub-critical fluid chromatography thin-layer chromatography... 

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