Autosampler, high-performance liquid chromatography

The principle was demonstrated using triazine herbicides as templates and by varying the type of functional monomer and the monomer composition. With a final batch size of ca. 40 mg of monomer, the consumption of monomers and template is significantly reduced and the synthesis and evaluation can take place in standard high-performance liquid chromatography (UPLC) autosample vials. After synthesis. 

Procedure Flavonoids are then further purified with 2 ml of methanolic HC1 (2 N), followed by centrifugation (2 min, 15 600 g), hydrolyzation of 150 il of suspension in an autoclave (15 min, 120 C). A reverse osmosis-Millipore UF Plus water purification system is used in high performance liquid chromatography (HPLC) with an autosampler. After injections of 5 pg of samples, the mobile phases flow at a rate of 1 ml/minute with isocratic elution in a column at 30 C. 

Fig. 6.1.4 Components of the high-performance liquid chromatography (HPLC) system for oxidized pterins with column switching. The autosampler starts the chromatography software, which controls valves and pumps. Pump 3 is used only for rinsing the precolumn. Details of the three valves and their different positions are explained in Fig. 6.1.5...

High-performance liquid chromatograph (HPLC e.g., Waters Chromatography) equipped with column heater, solvent pump, UV detector (set at 210 nm), integrator, autosampler, and (for manual injection) a 10-pl sample loop 15 x 0.46-cm YMC-ODS-AQ analytical column (AQ12S031546WT, Waters Chromatography)... 

Procedure (See Chromatography, Appendix BA.) Use a suitable high-performance liquid chromatograph equipped with differential refractometer, autosampler injection unit, mobile-phase degasser, column heating block or oven, and a computing integrator. The column is Lichrosorb RP-18 250-mm x 4.5 mm (id) (GL Science, Inc., or equivalent) and YMC-Pack ODA-A A-303 250-mm x 4.5 mm (id) (YMC Company, Ltd., or equivalent) connected in a series, or equivalent, maintained at 50°. Use 80 20 acetone acetonitrile as the eluent, at a flow rate of 2 mL/min. 

SEC was performed according to the method of Chin et al. (1994). A Shodex KW802.5 SEC column (Waters Corp., Milford, MA., USA) was used and a Waters liquid chromatography system consisting of the following components was used for the analysis Waters 501 high pressure pump. Waters 717 autosampler, InterAction column temperature control oven, Waters 484 UV/VIS detector, and Waters Millenium 2.0 computer software package. 

High-performance nanoscale liquid chromatography system (Eksigent [Dublin, CA] nano-LC or equivalent), equipped with autosampler IntegraFrit sample trap, 25 mm x 75 pm internal diameter (i.d.) (New Objective [Woburn, MA]) or equivalent preconcentration column PepMap C18 analytical column, 150 mm x 75 pm i.d., 3 pm particle size, 100 A pore size (LC Packings, San Francisco, CA) or equivalent nano-LC column packed with octadecylsiUca particles. 

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