High performance liquid chromatography assay preparation

Bendriss, E. Markoglou, N. Wainer, I.W. "High-Performance Liquid Chromatography Assay for Simultaneous Determination of Dextromethorphan and Its Main Metabolites in Urine and in Microsomal Preparations, J. Chromatogr. B 754, 209-215 (2001). 

Benson, H.A.E. McElnay, J.C. High-performance liquid chromatography assay for the measurement of benzyda-mine hydrochloride in topical pharmaceutical preparations. J. Chromatogr. 1987, 394 (2), 395-399. 

I. Papadoyannis, M. Georgarakis, V. Samanidou, and A. Zotou, Rapid assay for the determination of tolfenamic acid in pharmaceutical preparations and biological fluids by high-performance liquid chromatography, J. Liquid Chromatogr., 74 2951 (1991). 

System Suitability Chromatograph a suitable number of injections of the Assay Preparation, as directed under Calibration, to ensure that the resolution factor, R, between the major peaks occurring at retention times of approximately 0.50 (8-tocopheryl propionate) and 0.63 ((3- plus y-tocopheryl propionates), relative to hexadecyl hexadecanoate at 1.00, is not less than 2.5 (see System Suitability in High-Performance Liquid Chromatography under Chromatography, Appendix IIA). 

The determination of lovastatin and its hydroxyacid metabolite in plasma and bile can be accomplished by high performance liquid chromatography (25). Plasma samples are prepared for analysis by solid phase extraction and are analyzed using isocratic elution on a Cl 8 column. Bile samples do not require any sample dean-up prior to HPLC analysis, but do require the use of a gradient elution method to separate the compounds of interest. The HPLC assay has a limit of detection of 25 ng/mL... 

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