High-Performance Liquid Chromatographic Methods for the Determination of Aconitine Alkaloids

In chemical and pharmacological investigations of the Japanese drug sen-bushi , the roots of A. oavmiohaeli, Japanese workers S 

A report24 of the alkaloids of the roots of A. carmiahaeli-Debx. describes the isolation and structure elucidation of fuzi-line , C24H39NO7, m.p. 214-216 C., and the known bases aconitine (14), hypaconitine (12), mesaconitine (10), neoline (19), and song-orine (27). The alkaloid contents of these plants were found to vary considerably with growing location.25 From and n.m.r. 

Zhu and Zhu discussed the isolation of six alkaloids from the roots of A. oaemiohaeli (which they referenced as the traditional Chinese drugs Chuan-wu and Fu-tzu). Aconitine (14), hypaconitine (12), mesaconitine (10), talatizamine (15), isotalatizamine, and isotalatizidine (17) were identified. The base previously named chuan-wu base A is identical with isotalatizidine. 

spectral data for crassicauline B indicated the presence of two hydroxyl groups, a benzoyloxyl group, and an exocyclic double bond. These data indicated that this alkaloid is similar in structure, but not identical, with isohypognavine (34). 

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