High-performance liquid chromatographic HPLC methods

High-performance liquid chromatographic (HPLC) methods 

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Ciccolini J, Mercier C, Blachon MF et al. A simple and rapid high-performance liquid chromatographic (HPLC) method for 5-fluorouracil (5-FU) assay in plasma and possible detection of patients with impaired dihydropyrimidine dehydrogenase (DPD) activity. J Clin Pharm Ther 2004 29 307-315. 

A high-performance liquid chromatographic (HPLC) method has enabled the determination of thalicarpine and its metabolites hemandaline, hemandalinol, and dehydrothalicarpine, in urine (262). Thalmelatine, thalipine, and thalicarpine were quantitated in Thalictrum minus (335). HPLC methods for quantitation of tubocurarine in curare (335a) and in human plasma (335b,335c) have been described. 

EDTA has been determined in a wide variety of sample matrices by HPLC. These matrices include waste waters, natural waters, sediments, fertilizers, chemical cleaning solutions, radioactive waste solutions, and pharmaceutical preparations. Chinnick reported the separation and identification of EDTA and other aminopolycarboxylic acid sequestrants by a high performance liquid chromatographic (HPLC) method [57]. 

Body fluids are analyzed for T3 and T4 by a variety of radioimmunoassay procedures (31) (see Immunoassays). The important clinical parameter for estimating thyroid function, the protein-bound iodine (PBI), is measured as described in treatises of clinical chemistry. High performance liquid chromatographic (hplc) methods have replaced tic (32,33). 

Wood etal. (1988) developed the reversed-phase high-performance liquid chromatographic (HPLC) method for piperine determination in black pepper and its oleoresins. ft employs bonded C18 stationary phase (ODS-2) and acetonitrile-aqueous acetic acid mobile phase with UV detection. As the spectropho-tometric method which invariably yields higher results because of the contributions from other alkaloids such as piperyline and piperettine, the HPLC method relates more to piperine. Utilizing the UV absorption property of piperine, spectrophotometric estimation methods were developed by different groups using solvents such as benzene, ethanol, ethylene dichloride, acetone, ethyl acetate, chloroform and cyclohexane. Sowbhagya et al. (1990) proved that extractability with acetone was very efficient. 

High-Performance Liquid Chromatographic (HPLC) Methods... 

Cetin et al. [29] proposed a simple, precise, fast, and low-cost differential pulse (DP) polarographic method for fhe deferminafion of lornoxicam in pharmaceutical formulations. Results have been compared with those of high-performance liquid chromatographic (HPLC) method. Mean values and standard deviations calculated by 10 determinations were 8.10 0.12 mg for DP polarography and six determinations were 8.02 0.08 mg for HPLC mefhod. The sfafisfical evaluations indicated that there was no significant difference between the mean values and precessions of fhe fwo mefhods af 95% confidence level (f = 2.64, F = 2.25). 

Rates and products of solvolytic reactions (equation 1) were obtained by standard NMR or high-performance liquid chromatographic (HPLC) methods. Rates of reactions were also monitored from the appearance of acid (HX, equation 1), determined by the change in conductance of the solution or by titrimetric methods. For anions X- containing a suitable chromophore, reaction rates were monitored by the change in UV spectrum of the solution. The chemicals required for this study were either... 

Because of the high separation power, reliability, and reproducibility, various high-performance liquid chromatographic (HPLC) methods have been extensively employed for the separation and quantitative determination of a considerable number of compounds, including pharmaceuticals, food components, environmental pollutants. 

The next step was to develop technology to rapidly quantify each such that the load could be accepted/rejected in an acceptable time frame. Kupina (1984) developed a high-performance liquid chromatographic (HPLC) method that simultaneously quantified each of the msgor metabolites in 11 min. Briefly, the procedure involves chromatographing a composite filtered juice sample on a HPLC column which separates the three components from each other and other matrix compounds. Quantification is accomplished by comparing the component peak areas to those of a standard solution chromatographed in the same way. 

Attempts by Brodnitz et al. in 1971 to determine allicin by gas chromatography-mass spectrometry (GC-MS) provided evidence for an unusual mode of decomposition (70). They indicated that GC caused diallyl thiosulfinate to dehydrate according to the reaction C6HiqS20 -> C gS2 + H2O, affording a 2.4 1 mixture of two compounds, claimed (incorrectly as we shall see below) to be 3-vinyl-4H-l,2-dithiin (10, equation 1) and 3-vinyl-6H-l,2-dithiin (10a, equation 1) (70). Analytical methods for allicin quantitation that rely on GC detection of compounds 10 and 10a are still used (77), even though serious problems exist with this method and more accurate high-performance liquid chromatographic (HPLC) methods have been developed (see below). 

Quality components such carotenoids, ascorbic acid and tocopherol were analysed by high performance liquid chromatographic (HPLC) methods [ 3, 4, 5]. 

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