High imprinted chiral phases

Sellergren B. Imprinted chiral stationary phases in high-performance liquid chromatography. J Chromatogr A 2001a 906 227-252. 

Shea, K.J. Molecular imprinting of synthetic network polymers the de novo synthesis of macromolecular binding and catalytic sites. Trends. Polym. Sci. 1994, 2, 166-173. Sellergren, B. Imprinted chiral stationary phases in high-performance liquid chromatography. J. Chromatogr. A 2001, 906, 227-252. 

Sellergren B. Molecular imprinting by noncovalent interactions tailor-made chiral stationary phases of high selectivity and sample load capacity. Chirality 1989 1 63-68. 

Chiral CEC will be discussed in detail later in the book but is included here to exemplify the application of the high efficiencies obtained with electro-driven techniques which makes them attractive for chiral analysis where selectivity factors are sometimes small. CE has made use of chiral additives in the electrolyte whilst LC tends to utilise chiral stationary phases. Both options have been explored for chiral CEC [27,28,77]. The small amount of packing material necessary for capillaries allows the use of chiral stationary phases that would be prohibitively expensive for standard LC. Cyclodextrins, proteins, antibiotics and molecular imprinting have all been used to form chiral stationary phases [78-80]. After some less than encouraging peak efficiencies obtained using the chiral CEC approach, much improved chiral resolutions have been achieved using CEC compared to LC or CE [81-83]. 

The high selectivity of M IPs is demonstrated when an optically active compound is imprinted the resulting MIP will normally resolve the racemate. Numerous reports on MIP chiral stationary phases have appeared [184—188]. Chiral templates studied include amino acids [26, 29, 120, 139, 189-192], peptides [139, 192, 193], carbohydrates [58, 194, 195] and dmgs [127, 196]. 

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