Macromolecular binding

DREAM++ A set of programs (ORIENT++, REACT++ and SEARCH++) for docking computationally generated ligands into macromolecular binding sites University of California [31]... 

N-Glucuronyloxy arylamides do not appear to be important in hepatocarcinogenesis as their increased metabolic formation does not result in increased hepatic macromolecular binding (4,25). 

Phase-resolved, or phase-suppression technology was investigated for FIA development by McGown and co-workers(49, 53, 105, 106) to test the prediction that phase fluorometry would be useful for monitoring two-state equilibria in the analysis of macromolecular binding interactions)31,104) Fluorophores with different fluorescence... 

L.L. Sasmor, H. Griffey, R.H. Measurement of macromolecular binding using electrospray mass spectrometry. Determination of dissociation constants for oligonucleotide serum albumin complexes. J. Am. Chem. Soc. 1995, 117, 10765-10766. 

Ghanayem BI, Burka LT, Matthews HB Ethyl acrylate distribution, macromolecular binding, excretion and metabolism in male Fisher 344 rats. Fundam Appl Toxicol 9 389-397, 1987... 

Dybing, E., Soderlund, E.J. Nelson, S.D. (1980) Irreversible macromolecular binding of the flame retardant tris(2,3-dibromopropyl)phosphate in vitro and in vivo. Develop. Toxicol, environ. Sci., 8, 265-268... 

Gao, P., Thornton-Manning, J.R. Pegram, R.A. (1996) Protective effects of glutathione on bromodichloromethane in vivo toxicity and in vitro macromolecular binding in Fischer 344 rats. J. Toxicol, environ. Health, 49, 145-159... 

Modeling of Drug-Receptor Interactions. The identification of molecular interactions between drugs and their receptor or enzyme targets and the three-dimensional spatial requirements of the macromolecular binding pocket are important for the rational design of new, more selective. 

The simplest experiments are those that focus just on the ligand. These are typically used to determine solution conformations or 3D structures of ligands. Homonuclear1H II ) or 21) NMR experiments are used mainly here. At the other end of the scale, experiments to study the macromolecular binding partner often require labeled protein and multidimensional NMR methods, as indicated on the right-hand side of Fig. 1. Finally, many NMR experiments provide information... 

Application of a solution or dispersion of macromolecular binding agent onto a substrate and evaporation of the solvent. In order to reduce the amount of organic solvent, water based solutions and dispersions are being used more frequently. 

Glomerular filtration rate Skin-air Macromolecular binding constants... 

It appears that in most cases the techniques are comparable in terms of resulting sensitivity. Whereas the SPR has the advantage in terms of real sensitivity, the fact the QCM technique also measures entrapped water amplifies the gravimetric response and may render it sensitivity comparable in macromolecular binding experiments [44, 45]. An added advantage of QCM over SPR is the availability of the QCM-D technique which is a measurement of the dissipation energy. A film that is viscoelastic or soft will not fully couple with the quartz crystal s oscillation... 

Figure 10.15 Screening of the phage hbrary L6 to find macromolecular binding partners for pachtaxel identification of Bcl-2 as a binding partner.

Figure 10.23 Screening of the phage display libraries L12 and L13 to find macromolecular binding partners for intersection the selection/amplification process and the structuie of selected binding sequences 10.19-10.21 for the EH domains of intersectin.

M] is the macromolecular binding sites concentration [T] is the template concentration... 

Although there is substantial documentation regarding health effects of 1,3-dichloropropene, little data concerning mechanisms of toxicity are available. 1,3-Dichloropropene acts as an irritant and sensitizer. Macromolecular binding of this compound may also contribute to its toxicity. 

TNT increases UDPglucuronsyltransferase in the liver and kidneys. It increases renal epoxide hydrolase activity. Animal studies have suggested covalent binding between TNT and macromolecular proteins including serum albumin, hemoglobin (Hb), hepatic and renal proteins, and possibly lens protein. The Hb adduct was dose dependent. Macromolecular binding is likely to be correlated with toxic effects ... 

Receptors are macromolecular binding sites for low molecular weight molecules (ligands), such as hormones and neurotransmitters. As such they are crucial to the well-being of the body. Xenobiotic ligands bind with receptors and in doing so interrupt the normal functioning of the body. 

Gong K, Mailloux L, I ler/berg MC Salivary film expresses a complex, macromolecular binding site for Streptococcus sanguis. J Biol Chem 2000 275 8970-8974. 

Shea, K.J, Molecular imprinting of synthetic network polymers the de-novo synthesis of macromolecular binding and catalytic sites, Trends. Polym. Sci. 1994, 19, 9-14... 

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