Protein-heparin interactions

Capila I, Linhardt RJ (2002) Heparin - Protein interactions. Angew Chem Int Ed 41 391—412. 

Heparin protein interactions. In Lane DA et al (eds) Heparin and related polysaccharides. Plenum Press, New York, p 141... 

Capila I, Linhardt RJ Heparin-protein interactions. Angew Chemie Int. Ed. (2002)... 

Noti C, de Paz JL, Polito L, Seeberger PH. Preparation and use of microarrays containing synthetic heparin oligosaccharides for the rapid analysis of heparin-protein interactions. Chem. A Euro. J. 2006 12 8664-8686. 

Cardiogenic shock can occur in parallel with disseminated intravascular coagulation (5). In these circumstances heparin is thought to act as a hapten in a heparin-protein interaction that stimulates antibody production and an antigen-antibody reaction associated with release of platelet and vasoactive compounds. 

Capila I, Linhardt RJ (2002) Heparin-protein interactions. Angew Chem Int Ed 41 391 12 Carmeliet P (2003) Angiogenesis in health and disease. Nat Med 9 653-660 Chen CP, Imanishi Y, Ito Y (1998) Effect of protein and cell behavior on pattern-grafted thermoresponsive polymer. J Biomed Mater Res 42 38-44 Cheng XH, Canavan HE, Stein MJ et al (2005) Surface chemical and mechanical properties of plasma-polymerized N-isopropylacrylamide. Langmuir 21 7833-7841 Curti PS, de Moura MR, Veiga W et al (2005) Characterization of PNIPAAm photografted on PET and PS surfaces. Appl Surf Sci 245 223-233... 

Capila, I. and Linhardt, R. J. Heparin-protein interactions. Angew Chem Int Ed Engl, 41, 391, 2002. 

Capda, I., Linhardt, R. J. (2002). Heparin-protein interactions. Angewandte Chemie International Edition in English, 41(3), 391—412. Retrieved from, http //www.ncbi.nlm.nih. gov/entrez/query.fcgi cmd=Retrieve db=PubMed dopt=Citation list uids=12491369. 

Interactions can also be studied at the surface of a coated capillary wall. One binding partner is first immobilized on the capillary wall. As a result of the affinity of the second binding partner, the analyte will be delayed, compared with migration times observed in an untreated capillary. Based on this approach, modified capillaries have been prepared and used successfully to study polysaccharide-protein interactions as well as affinity separations. Coating of the capillary wall with heparin and heparan sulfate has been used to determine the affinity of these polysaccharides for synthetic heparin-binding peptides different only in the stereochemistry of a single... 

As polyanionic saccharides, heparinoids interact with a high number of proteins, the so-called heparin binding proteins. Heparinoid - protein interactions have usually been described for in vitro systems, and their physiological relevance is not always clear. On the other hand, heparinoids can influence biological systems, but in most cases the molecular basis is not fully understood. For this reason, interactions with proteins and biological properties of heparinoids will be discussed separately in the following. As heparinoid - protein interactions have been reviewed [3] the focus will be in rather short form on those areas in which heparinoid mimetics have been investigated. 

Fig. 5. Hidden protein-binding sequences in HS. (o) Two different proteins bind to the same, fully sulfated ( regular ) heparin sequence. (Z>) The same proteins interact specifically each with one distinct HS sequence. The symbols are the same as in Fig. 1. For further information see the text.

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