Headspace solid phase microextraction SPME

GC combined with mass spectroscopic (MS) detection provides very accurate identification and quantification of FFAs. Pinho et al. (2003) monitored changes in the FFA content during the ripening of ewe cheese. Sampling was done by headspace solid-phase microextraction (SPME). An excellent correlation was observed between the initial concentration of the sample and the amount absorbed on the SPME fiber. SPME sampling was done at 65 °C with a fiber coated with 85-p.m polyacrylate film. After equilibration at 65 °C for 40 min, the fiber was exposed to the sample headspace for 20 min and inserted into the GC port. Despite its accuracy, the GC-MS method is not widely used, presumably because of its cost and complexity. 

Lecanu, L., Ducrest, V., Jouquand, C., Gratadoux, J. J., and Feigenbaum, A. (2002). Optimization of headspace solid-phase microextraction (SPME) for the odor analysis of surface ripened cheese. ]. Agric. Food Chem. 50, 3810-3817. 

K. Takekawa, M. Oya, A. Kido and O. Suzuki, Analysis of cyanide in blood by headspace solid-phase microextraction (SPME) and capillary gas chromatography, Chromatographia, 47, 209-214 (1998). 

The most useful method for solvent residue analysis is GC. It can be performed by direct injection technique, or by headspace, solid phase microextraction (SPME), or single-drop microextraction (SOME) techniques [96]. GC has high selectivity, good specificity, is easy to perform, and involves simple sample preparation. Modem capillary GC allows separation of many compounds, together with their identification and quantification [96]. GC uses different detector systems, which are presented in Table 8.7. 

Prison, G.,Tedeschi, L, Maietti, S. and Ferrara, S.D. (1998). Determination of gamma-hydroxybutyric Acid (GHB) in plasma and urine by headspace solid-phase microextraction (SPME) and gas chromatography-positive ion chemical ionization-mass spectrometry. In Proceedings of the 1998 Joint Society of Forensic Toxicologists and the International Association of Forensic Toxicologists SOFT/TIAFT International Meeting, Spiehler, V. (Ed.), pp. 394 404. 

Headspace solid-phase microextraction (SPME)-GC/MS Direct analysis of total lipid extracts... 

Use of headspace solid-phase microextraction (SPME) LOD = 0.006 pg/mL Determined as the pentafluorobenzylbromide derivative in the presence of TDMBC... 

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