Haptens hemagglutination inhibition

In indirect or passive hemagglutination, the erythrocytes are used as a particulate carrier of foreign antigen (and in some tests of antibodies) this technique has wide applications. Other materials available in the form of fine particles, such as bentonite and latex, also have been used as antigen carriers, but they are more difficult to coat, standardize, and store. In a related variation of tliis technique, known as hemagglutination inhibition, the ability of antigens, haptens, or other substances to specifically inhibit hemagglutination of sensitized (coated) cells by antibody is determined. 

The basis of the radioimmunoassay is similar to that for hemagglutination inhibition, in that an antigen (or in radioimmunoassay it may be a hapten) is labeled, and used to compete, quantitatively, with unlabeled hormone (standard or test) at the antibody surface. 

Studies on the carbohydrate-binding specificity of the fava-bean lectin, as determined by hapten inhibition of hemagglutination,140,213,466-469 showed that the lectin is inhibited by Makela s group III sugars (see Table VI). Hemagglutination by the lectin was inhibited by... 

B. DETERMINATION OF CARBOHYDRATE SPECIFICITY BY HAPTEN INHIBITION OF HEMAGGLUTINATION... 

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