Hanes’ reagent

The most effective solvent is sodium hydrogenphosphate, if the detection is not done with phosphate reagent (e.g., Hanes reagent see below). 

For identification of phospholipids use Hanes reagent as described in Protocol 3.1.4. 

In high-voltage paper-electrophoresis with 3 1 36 acetic acid-formic acid-water under 3.50 kV for 15 minutes, the inactivated streptomycin moved 10.5 cm towards the cathode and streptomycin moved 13.0 cm, indicating that the inactivated streptomycin is less basic than streptomycin. It gave a positive reaction with the Hanes reagent. A solution of the inactivated streptomycin in distilled water showed a u.v. maximum at 260 nm. Determination of streptomycin by the maltol reaction (by use of the optical absorbance at 550 nm) indicated the presence of streptomycin and adenylic acid in the equimolar ratio of 1 1. The empirieal formula of monoadenylylstreptomycin dihydrochloride tetrahydrate was shown by elementary analysis. The inactivated streptomycin was hydrolyzed by phosphate diesterase" to streptomycin (14) and 5 -adenylic acid. 

Molybdate(ammoninm)-perchloric acid (Hanes reagent) for phosphate esters... 

Certain other sulfur-containing reagents also cleave methyl and other ethers see Hanes-sian, S. Guindon, Y. Tetrahedron Lett., 1980, 21, 2305 Williard, P.G. Fryhle, C.B. Tetrahedron Lett., 1980, 21, 3731 Node, M. Nishide, K. Fuji, K. Fujita, E. J. Org. Chem., 1980, 45, 4275. For cleavage with selenium-containing reagents, see Evers, M. Christiaens, L. Tetrahedron Lett., 1983, 24, 377. For a review of the cleavage of aryl alkyl ethers, see Tiecco, M. Synthesis, 1988, 749. 

C-3 (or C-4) from a 1,3-dioxolane actually, this reaction (see Section HI, 14) was observed for 2,2-disubstituted 1,3-dioxolanes, and it may be assumed that, if there is any competition between removal of a proton from C-2 and from C-3 (or C-4), the rate of the first reaction is far greater than that of the second. The possibility of dioxolanylium ions reacting with nucleophilic reagents was first demonstrated by Hanes-sian and Staub,152 and the scope of these reactions was independently investigated by Pedersen and coworkers.153-157 Hanessian and Staub152 treated methyl 5-0-benzoyl-2,3-0-benzylidene-j3-D-ribofuranoside (125) with triphenylmethyl fluoroborate in acetonitrile, and obtained, after hydrolysis, a 1 1 mixture of the two benzoates 127 and 128 (yield 83%), probably through the benzoxonium ion 126. The same reaction... 

Aniline-acid-oxalate spray reagent (see Appendix) Modified Hanes-Ischerwood spray reagent [(4% (NH4)6Mo024 4 H20 1 N HC1 70% HC104 H20 (5 2 1 12)]... 

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