Reagent Hamilton

Proteins have been hydrolyzed by treatment with sulfuric acid, hydrochloric acid, barium hydroxide, proteolytic enzymes, and other hydrolytic reagents, but no condition has been found which avoids some destruction or incomplete liberation of tryptophan, cystine, and some other amino acids. The early work on this problem has been reviewed by Mitchell and Hamilton (194). The literature and their own excellent experiments on the hydrolysis problem in relation to the liberation and destruction of tryptophan have been presented recently by Spies and Chambers (269). 

An improved high pressure liquid chromatographic (HPLC) procedure Tor the PSP toxins is described. The method involves separation of the toxins on a polystyrene divinylbenzene resin colunn (Hamilton, PRP-1) in the reversed phase mode using heptane and hexane sulfonic acids as ion-pairing reagents. Detection of the toxins is by fluorescence following post-column alkaline periodate oxidation. The sensitivity of the HPLC method is better than the standard mouse bioassay by at least a factor of four for each of the individual toxins. 

Toxin Separations. A number of columns have been evaluated for suitability using the HPLC method. Of all columns tested to date, the Hamilton PRP-1 column (polystyrene divinylbenzene resin) has proven to be the most useful. Toxin retention on this column is controlled by 1) methanol concentration, 2) mobile phase ionic strength, 3) chain length of the ion-pair reagent, and M) mobile phase pH. 

J. S. Albert, A. D. Hamilton, Encyclopedia of Reagents for Organic Synthesis, John Wiley and... 

Patel, D. J. Hamilton, C. L. Roberts, J. D. Small ring compounds. XLIV. Interconversion of cydopropylcarbinyl and allylcarbinyl Grignard reagents./. Am. Chem. Soc. 1965, 87, 5144-5148. 

The most simple and common workstations are those for dilution and/or reagent addition to a number of samples in a simultaneous manner, either to all samples in a rack or to a line with a slide z-axis (as in the Biomex" 2000 model from Beckman). Most workstations are designed to operate with liquid samples such is the case with those from Cyberlab, Gilson, Zymark, SciLog, Sagian, Beckman and Hamilton, which manufacture specific equipment for liquid handling, solid-phase extraction and preparation of liquid... 

The system uses high-precision Hamilton pipettes to transfer samples (3-20 pl ) and reagents (300 pL) to the central reaction tray. Some inert fluid TRAF, the instrument s most significant innovation, is used to avoid carryover. Each parameter is measured In a single cuvette thus, if five parameters per sample are to be determined, the instrument capacity will be 20... 

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