H Antigens

Peptides formed during tryptic digest of Salmonella flagellin were immobilized on the WPG-PG to prepare immunoadsorbents for the isolation of monoreceptor antibodies from rabbit serum against H-antigens of Salmonella spp. [129]. The... 

Ohta, M., Matsuura, F., Kobayashi, Y., Shigeta, S., Ono, K., and Oka, S., Further characterization of allergenically active oligosaccharitols isolated from a sea squirt H-antigen, Arch. Biochem. Biophys., 290, 474, 1991. 

Ino H. Antigen retrieval by heating en bloc for pre-fixed frozen material. /. Histochem. Cytochem. 2003 51 995-1003. 

The efficiency of this approach could be demonstrated in a-fucosylation with donor 32a and the acceptors 19C and 33A-D (Table XXXIII). Thus, with the help of this inverse procedure, the versatile building blocks for syntheses of the Lea, Le, Ley, and H antigen determinants are readily accessible (176). Presumably, this procedure may become of general importance when reactive glycosylating agents are employed. Alternatively, the reactivity of the fucosyl donor could be decreased, as has been recently proven very successfully (177). 

SOLID-PHASE SYNTHESIS OF THE HEXASACCHARIDE GLOBO-H ANTIGEN PROGRESS AND LIMITATIONS... 

The ABO antigens are oligosaccharides attached to membrane proteins or lipids. The H antigen (carried on blood group O cells) is the biochemical... 

Other enzymes alter them by hydrolytic removal of sugars, by isomerizaion, oxidation, and addition of other components such as phospho, sulfo, and acetal groups. For example, the presence of the H-antigen determinant, whose structure is shown in Box 4-C, is strictly regulated, both temporally and spatially, during vertebrate development.215 216 The relative amounts of the H determinant vs Lex, sLex (Box 4-C)... 

Maurer, P. H. Antigenicity of polypeptides (poly-alpha-amino acids). XVII. Immunologic studies in humans with polymers containing L or D and L-alpha-amino acids. J. Immunol. 95 1095-1099, 1965. 

G. Schiffman, E. A. Kabat, and W. Thompson, Immunochemical studies on blood groups. XXXn. Immunochemical properties of and possible partial structures for the blood group A, B, and H antigenic determinants, Biochem. J., 3 (1964) 587-593. 

F6. Pemice, W., Sosomann, C., Luben, G., Seiler, F, R., and Sedlacek, H. H., Antigen-specific detection of HBsAg-containing immune complexes in the course of hepatitis B virus infection. Clin. Exp. Immunol. 37, 376-380 (1979). 

NuMA Nuclear mitotic apparatus protein SP-H antigen... 

The descriptive phase of biology is an essential prerequisite for the healthy growth of biochemistry, and the serological classification of the Salmonellas and related Enterobacteria has allowed a comprehensive and reproducible series of analyses to be made where otherwise a chaotic situation could have arisen. The Kauffmann-White Schema embraces more than the specificities due to polysaccharides of the O antigens. The H antigens, for example, are specificities due to the protein components of the flagella, and these are probably beyond the scope of chemical methods available at this time. 

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