Growth of Bacteriophage

Hershey, A. D., and M. Chase, Independent functions of viral proteins and nucleic acid in growth of bacteriophage. 

The steroidal diamines, irehdiamine-A (244) (IDA) and malouetine (243) (MAL), exert an inhibiting action on the growth of bacteriophage T2 in infected cells of Escherichia coli. Bacteriophage-directed DNA synthesis is more sensitive to steroidal amine inhibition than RNA or protein synthesis, and it is proposed that DNA is the primary target of steroidal diamine action. 

Rutberg, L., Rutberg, B. Growth of bacteriophage 0105 and its deoxyribonucleic acid in radiation-sensitive mutants of Bacillus suhtilis. J. Virol. 8, 919-921 (1971). 

Restriction endonuclease A deoxyribonuclease which cuts DNA at specific sequences which exhibit twofold symmetry about a point. Name derives from the fact that their presence in a bacterial cell prevents (restricts) the growth of many infecting bacteriophages. 

Reiter et al. (1964) showed that growth of S. aureus in raw, steamed, and pasteurized milk was inhibited by a lactic starter culture. When they neutralized the lactic acid as it was produced, inhibition of the staphylococcus was still evident. Jezeski et al. (1967) also observed that growth of S. aureus in steamed or sterile reconstituted nonfat dry milk was inhibited by an actively growing S. lactis culture. Enter-otoxin was detected in S. aureus-S. lactis mixed cultures when S. lactis was inactivated by bacteriophage but not when the lactic streptococcus grew normally. Further information on S. aureus has been summarized by Minor and Marth (1976). 

Usually, the detection of pathogenic bacteria, such as Escherichia coli is based on the selective growth of these bacteria in liquid media or on plates. This procedure may require several days [52]. More recently, methods such as pathogen recognition by fluorescently labeled antibodies, DNA probes, or bacteriophages have been developed and proved to be much faster [52],... 

Plaque - A cleared area on a Petri dish on a plate of bacteria arising from growth of a bacteriophage in that region. 

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