Growth agents

In earlier investigations by the author, nerve growth agents consisting of 1,2,3,4-tetrahydro-isoquinoline, (I), (3), and azo-amino acid derivatives, (II), (4), were prepared and used in the treatment of neurological disorders. 

Estrogen antagonists consisting of 4-fluoroalkyl-2H-benzopyrans, (IV), and 3,4-dihydroquinoline derivatives were prepared by Kuenzer (5) and Wallace (6), respectively, for use as antiuterus growth agents. 

Cells are plated at the desired density, either on sterile glass cover-slips for microscopic examination, or directly into multiwell plates (prepare each point at least in tripUcate) for population analysis by ELISA. After treatment of the cells with the desired growth agents, BrdU is added, following the same criteria already discussed for thymidine labeling (short pulse, or long term). Cells are then washed free of residual BrdU by rinsing with PBS, and fixed in ethanol. Fixed cells can be stored at 4°C for further analysis. BrdU... 

Almasio, R, Piovella, F., Ricetti, M. M., Samaden, A. and Semino, G. (1984). ECMs produced in the presence of dexamethasone a natural growth agent for human cultured endothelium. Boll. Soc. Ital. Biol. Sperim. 60, 225-230. 

Western-style psychotherapy is limited because it is likely to be used not on structures that are basic to the samsaric condition, but only on structures that produce experiences and behaviors that are not acceptable in the particular consensus reality. Thus many psychotherapists are not growth agents in a general sense, but rather work to readjust a deviant person to the consensus reality of his culture. This is not a conscious manipulation on the part of these psychotherapists, but simply a reflection of the power and implicitness of their own enculturation processes. Psychotherapy can be a subversive tool in some practitioners hands, for some of the assumptions of the consensus reality can be questioned in it, and the patient can grow beyond his culture in some ways. All too often, however, the implicit assumptions are not even questioned. 

Plant, N. J., Horley, N. J., Dickins, M., Hasmall, S., Elcombe, C. R., and Bell, D. R. (1998). The coordinate regulation of DNA synthesis and suppression of apoptosis is differentially regulated by the liver growth agents, phenobarbital and methylclofenapate. Carcinogenesis 19, 1521-1527. 

Microencapsulation is also of importance in agricultural applications. Encapsulation of fertilizers ensures their slow release into soil and more uniform feeding to plants. Microencapsulation allows one to coat the surface of seeds with various important substances, such as growth agents, pesticides, and fertilizers. 

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