Tissue ground

The extraction of colorless catabolites from samples is usually performed by homogenizing the ground tissue in 20 to 100 mM K3PO4 buffer (pH 7.0) and methanol (1 1, v/v) - or 0.1 M Tris-HCl, pH 8.0 and methanol (1 4 v/v) followed by centrifugation and analysis by RP-HPLC, either directly or after concentration on a C18 SepPak cartridge. A reversed phase system with a C18 Hypersil ODS... 

Biopsy or blood specimens may be cultured for Leishmania spp. or T. cruzi with Novy-MacNeal-Nicolle (NNN) medium. Biopsy specimens are ground in a small amount of saline. Biopsies from skin lesions or other tissues which may contain bacteria may have penicillin (0.1 ml of 1,000 U/ml) added to the medium with the inoculum. The inoculum is 1 drop of ground tissue or blood. Incubate the culture at room temperature (22°C), and at days 3 and 7, examine a direct mount of liquid from the bottom of the slant at x400 magnification. These cultured organisms are potentially infective for humans. 

Simeonidou et al. (238) reported an ion-pair liquid chromatographic method for the determination of sulfadiazine, sulfamethazine, sulfadimethoxine, and sulfaquinoxaline residues in chicken muscle. According to this method, a 3 g ground tissue sample is extracted with 30 ml chloroform. Following centrifugation the supernatant is filtered and a 10 ml aliquot is extracted with 1 ml 3N hydrochloric acid and submitted to precolumn derivatization with fluorescamine. Liquid chro-... 

Table 4.1.12A. Herring meal (IFN 5-02-000). Fishmeal is the clean, dried, ground tissue of undecomposed whole fish or fish cuttings, either or both, with or without the extraction of part of the oil. If it bears a name descriptive of its type, it must correspond thereto. (From AAFCO, 2005.)...

A healthy root system functions to absorb water and dissolved chemicals and translocate them to the above-ground tissues. Many crop growth regulators, hormones, and other chemicals crucial to providing biotic and... 

There are three types of tissue that make up all of these plant parts. The dermal cells are the densely-packed outer layer, which varies in thickness in different plants. The cells in this layer may secrete a waxy coating or cuticle that helps reduce the amount of water lost through a plant s exposed surfaces. The ground tissue is the body of the plant. And vascular tissue is made up of the specialized cells that transport water, minerals, food, and hormones throughout the plant Vascular cells are divided into xylem, which transport water and minerals from roots to the upper plant, and phloem, which takes food (sugars) from the leaves to the rest of the plant. These two types of cells are usually arranged in parallel, concentric form, with the phloem on the outside. 

Fig. 4.3 Immunohistochemical analysis in Brassica napus (A,C,E) and Arabidopsis thaliana (B,D) of myrosinase by use of the antibody 3D7. Myrosinase is present in the ground tissue of cotyledons (left) and radicle (upper right) of B. napus embryo (A), while in A. thaliana seed no expression is detected (B) some cells in the seed coat retain unoxidized substrate. Arrows in (A) show examples of myrosinase-containing myrosin cells. In A. thaliana leaves, myrosinase is located in phloem myrosin cells (D), whereas in B. napus leaves myrosinase is in myrosin cells in the phloem (C), as well as in the ground tissue (E). For experimental details, see1. The size bars equal 100 pm.

The ESR spectrum of aerobically perfused rat heart with the frozen tissue chopped shows a symmetrical one-line spectrum, whereas ground tissue resulted in the appearance of a multi-line signal, a semiquinone at g = 2.004 and a peroxyl radical at gj = 2.006 and gy = 2.04 (Fig. 2) [75], The fact that radicals can be generated artifactually by mechanical grinding renders this procedure unsuitable for studying radical production during myocardial ischemia and... 

Marmots are herbivores, eating the above-ground tissues and tubers of a wide range of herbaceous plants, as well as buds, flowers, leaves, and young shoots of shrubs. They store food in their dens, some of which is consumed during the wintertime. 

Cross-section of cornstalk stem a, epidermis , cortex and c, ground tissue. After Stevens.)... 

A 50-g ground tissue sample was homogenized twice with 100 mL of methanol. The homogenates were centrifuged and the two supernatants combined. A 10 mL aliquot of the extract was diluted with 2 mL of water, the methanol evaporated, and an additional 5 mL of water added. The aqueous sample was adjusted to pH 10.5 with 100 iL of 2 M sodium bicarbonate and extracted twice with 10 mL of ethyl acetate. The ethyl acetate was evaporated to dryness, the residue dissolved in 5 mL of acetonitrile/methanol (9 1), and applied to a Bond Elut acid washed silica cartridge (Analytichem International, Harbor City, CA). After washing the cartridge with small amounts of acetonitrile/methanol (9 1), methanol, and dichloromethane, die ractopamine HCl was eluted with 8 mL of dichloromethane/methanol/triethylamine (84 15 1). The eluate was evaporated to dryness in a rotary vacuum evaporator, the residue dissolved in 2 mL of the HPLC mobile phase, and analyzed by HPLC. 

FIGURE 1. Above-Ground Tissue Comparison of Festuca Cultivars (at 10 weeks). 

---