Greening morphological changes

Kobayashi, M., Kakizono, T, and Nagai, S., Astaxanthin production by a green alga. Haematococcus pluvialis accompanied with morphological changes in acetate media, J. Perm. Bioeng., 71, 335, 1991. 

However, at the introduction of C60/PVP complex (up to 5 pg/ml) into the cultivating media during cell growth no effects were observed. In the cell MA-104 (cell line derived of green monkey kidney epithelium) grown normally within 3-6 days, no morphological changes and cell metabolism intensiveness were observed (Table 7.1). 

Fig. 8. Morphological changes of apoptotic eosinophils induced by dexamethasone (Z2). After eosinophils were treated (a) without or (b) with dexamethasone (2 /u,M) for 12 h, cells were harvested and detected by TUNEL assay using the In Situ Cell Death Detection Kit (Boehringer Mannheim). Briefly, cells were fixed with 4% paraformaldehyde and permeabilized by proteinase K and incubated with the TUNEL reaction mixture containing terminal deoxynucleotidyl transferase (TdT). After washing to remove unbound enzyme conjugated antibody, the horseradish peroxidase retained in the immune complex was visualized by a substrate reaction with diaminobenzidine. The cell nucleus was counterstained with methanol green. Apoptotic eosinophils with nuclear DNA breaks were seen to stain dark brown using a Nikon Eclipse E800 microscope (Nikon Corporation, Tokyo, Japan) in Fig. 8b.

Other laboratories have also carried out simultaneous measurements of the lipid or lipid metabolism changes associated with pyridazinone-induced in plastid morphology. Davies and Harwood examined the effects of Sandoz 6706 and Sandoz 9785 on lipid metabolism and membrane structure in greening barley. Changes seen in chloroplast structures included a slowing of normal differentiation on greening and the appearance of large... 

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