Green fluorescent protein three-dimensional structure

The combination of the capability of in vivo fluorescence video microscopy with the power of resolution of electron microscopy (EM) has been described. This approach is based on such an association of two techniques. An individual intracellular structure can be monitored in vivo, typically through the use of markers fused with green fluorescent protein (GFP), and a snapshot of its three-dimensional (3-D) ultrastructure and especially tomographic reconstruction can then be taken at any chosen time during its life cycle. The pitfalls and potential of this approach are discussed. 

Figure 2. Three-dimensional structure of Green Fluorescent Protein (GFP)...

The entire three-dimensional biofilm structure was imaged using MRI. This was then correlated to a fluorescent CLSM image by employing a green fluorescent protein reporter construct of S. oneidensis. Non-invasive techniques which enable measurements of dynamic metabolic processes, especially in a depth-resolved fashion, are expected to advance our understanding of processes occurring within biofllm communities. 

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