GraphPad Prism

Fig. 8.6 In vitro bioassay ofT] generation chloroplast transgenic line against P. aeruginosa. Bacterial cells from an overnight culture were diluted to A6oo 0.1 -0.3 and incubated for 2 hours at 25 °C with 100 xg of total protein extract. One ml of LB was added to each sample and incubated overnight at 26 °C. Absorbance was recorded at 600 nm. Data was analyzed using a GraphPad Prism.

Historically, data have been transformed to facilitate plotting on linear plots such as Lineweaver-Burk (1/y versus 1/[S]), Hanes-Woolf ([S]/y versus [S]), or Eadie-Hofstee (v/[S] versus y). However, with the present availability of affordable nonlinear regression and graphing software packages such as GraphPad Prism,... 

Further drawbacks associated with the direct linear plot include the fact that this analysis does not readily lend itself to standard computerized graphing methods (for example, use of GraphPad Prism), although specialized software is available (Henderson, 1993). Of course, one of the major advantages of the direct linear plot is the ability to obtain kinetic constants by eye, without the need for a computer. However, for presentation purposes, the use of graphing software is still desirable. Furthermore, any behavior more complicated than simple, single substrate kinetics - for example, turnover in the presence of an inhibitor, or multisubstrate kinetics - caimot readily be shown on a direct linear plot. This is in contrast with the flexibility afforded by nonhnear regression approaches. 

Fig. 2. CD154 and IL-4 stimulation of CLL cells Inhibits dmg-Induced cell death. CLL cells were cultured on WT and CD154 NIH3T3 fibroblasts using either the continuous (a) or the discontinuous (b) protocol In the presence of bortezomlb (30 nM), PHA-767491 (10 pIVI), fludarablne (10 p.M), or vehicle control for 24 h. Cell death was analyzed using a BD FACSCanto I flow cytometer (BD Biosciences) using annexin V/Pi staining. Dataware analyzed using FlowJo ( Tree Star) and GraphPad Prism software.

Wells JW (1992) In Hulme EC (ed.) Receptor-ligand interactions a practical approach. IRL Press, Oxford, p 289 Motulsky HJ (1999) GraphPad Prism, version 3.0. GraphPad Software, San Diego, p 303... 

The lipase activity unit (LU) is described in publication AF 95.1/2GB obtainable from Novo Nordisk A/S. The experimental data was fit to Eq. 6.1 with the non-linear regression program GraphPad Prism. 

Dose-response curves for PCB standard solutions are carried out. The curves are estimated by nonlinear regression using the following logistic equation [35] by GraphPad Prism 4 program ... 

Figure 3 Determination of S. pneumoniae MurD kinetic parameters. Initial velocities as a function of substrate concentration were measured using the ADP coupled enzyme assay with PK and LDH. Data were fit with a nonlinear regression analysis using GraphPad Prism. Top panel is for UMA and bottom panel is for d-G1u.

All data were presented as mean SEM. The data were evaluated statistically for differences by one-way analysis of variance (ANOVA) followed by post hoc Tukey test for multiple comparisons, p < 0.05 was regarded as significant. Calculations were done using GraphPad Prism. 

GraphPad Prism software (San Diego, CA) to a sigmoidal dose-response curve with variable slope model. Data points are the mean values and standard deviations are indicated with error bars (data from reference [89]). Reproduced with permission from (2006) Analytical Chemistry, 78, 3659-66. Copyright 2006 American Chemical Society. 

Motulzki, H.J. (1999) Analyzing Data with GraphPad Prism, GraphPad Software,... 

The changes of observed parameters were evaluated using repeated-measures ANOVA with Bonferronis corrected J>ost hoc r-test for multiple comparisons of dependent variables. Pearsons correlation coefficient and test were used for the evaluation of dependence of quantitative variables, -values less than 0.05 were considered significant. All statistic analyses were performed using the software package GraphPad Prism 4.0. 

Our statistical analysis and significance of static s calculation are performed using GraphPad Prism (GraphPad Sofiware). 

Add 50 pL/weU of H2SO4 2 M to stop the reaction. Read immediately the absorbance at 450 nm in an appropriate device. Data should be analyzed using GraphPad Prism nonhnear regression curve fitting or an equivalent software. 

GraphPad Prism v5.0 software was used to analyse the binding data from the above titrations. Plotting the change in ratio, AR(460 nm/520 nm), against ligand... 

All interactive mixtures were measured in triplicate and results were given as mean of three standard deviation. A paired t-test was performed for the results gained by Monckedieck et al. [11] to check for significance between the FPFs of the powder blends (GraphPad Prism, Version 6, Graph Pad Software Inc., La JoUa, USA). 

All statistical computations were performed using GraphPad Prism version 4.0a for Mac OS X (GraphPad Software, San Diego, California, USA). Values of experimental groups are shown as mean SEM unless otherwise stated. One-way ANOVA with Tukey s post-test analysis was used to determine statistical significance. Where appropriate, either two-tailed t-tests or Mann Whitney U tests were performed. A probability of P < 0.05 was considered to be statistically significant. 

These doses were given in 4.5 pi of petroleum ether. Numbers are means (95% confidence limits) of the dose estimated using the equation PR = 1/(1 + logdose) K /i) Yvhere PR is the proportional response and h the Hill slope using GraphPad PRISM 6.02. The probit slope was calculated... 

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