GraphPad Software

Finally, the 14 value for each FRAP experiment can be calculated as the time taken for half of the unbleached proteins to recover in the bleached region using PRISM Version 4 software (GraphPad Software). Comparing the Vi values for the same molecules under different experimental conditions can be used to determine the steps limiting the exchange rate. 

For each compound, means standard error (SEM) were calculated and differences were assessed by ANOVA. Calculated p values < 0.05 were considered to be significantly different. The statistical procedures were performed with the software programme Instat Version 3 (Graphpad Software Inc.). 

Wells JW (1992) In Hulme EC (ed.) Receptor-ligand interactions a practical approach. IRL Press, Oxford, p 289 Motulsky HJ (1999) GraphPad Prism, version 3.0. GraphPad Software, San Diego, p 303... 

GraphPad Software for analyzing binding data. Provides an introduction to analysis of ligand-protein interactions. http //www.chem.umd.edu/biochem/jollie/prob sets/set2 sols.htm... 

Data are reported as means S.E.M. and statistical analysis was performed by the Student s t-test or by ANOVA followed by Dunnett s post hoc test, as appropriate. Experimental data were elaborated by means of Prism 3 program (GraphPAD Software for Science, San Diego, CA, USA), and differences were considered statistically significant for P < 0.05. 

Motulzki, H.J. (1999) Analyzing Data with GraphPad Prism, GraphPad Software,... 

Fig. 4. Acyl-CoA binding assays. BARS was incubated with [ Cj-pahnitoyl-CoA and increasing concentrations of unlabeled acyl-CoAs or CoA, as indicated. The amount of radiolabeled pahnitoyl-CoA that remained bound to the protein was then quantified by gas ionization (Instant Imager, Packard Instrument, CT, USA) and the data were analyzed and plotted with the help of Prism (Graphpad Software). Representative experiment performed in duplicate.

All statistical computations were performed using GraphPad Prism version 4.0a for Mac OS X (GraphPad Software, San Diego, California, USA). Values of experimental groups are shown as mean SEM unless otherwise stated. One-way ANOVA with Tukey s post-test analysis was used to determine statistical significance. Where appropriate, either two-tailed t-tests or Mann Whitney U tests were performed. A probability of P < 0.05 was considered to be statistically significant. 

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