Gram-positive bacteria Bacillus megaterium

The Gram-positive bacteria Bacillus subtilis, Bacillus megaterium and Bacillus brevis are also used for heterologous expression. Terpe [13] presented a table with some pharmaceutically and industrially relevant proteins expressed with this host. It is the most used prokaryotic host after... 

Oxeta.nocins, Oxetanocia A (49), formerly oxetanocia, is the first naturally occurring oxetanose derivative and is isolated from Bacillus megaterium (1,145). It inhibits gram-positive bacteria, herpes vimses, and human immunodeficiency vims (HIV) (146). The chemical synthesis of (49) and several derivatives has been reported (147). 

Poly(hydroxybutyrate) was first discovered in 1926 as a constituent of the bacterium Bacillus megaterium (7). Since then, poly(hy-droxybutyrate) has been found in a wide variety of different genera of gram-negative and gram-positive bacteria (8). 

The production of B12, by bacteria, and the essentiality of B12 in both gram-negative and gram-positive bacteria, imply that B12 is a fundamental metabolite of bacteria. As with other organisms, it is unknown whether Co has functions other than in B metabolism. The Co required for B12 production for Bacillus megaterium was calculated by Garibaldi et al. (1953), who plotted B12 production as a function of Co concentration. From extrapolation to zero cobalamin, it appears that the purified culture medium had 0.4 /ig. Co per hter addition of Co to this medium did not increase growth. The ratio for biosynthesis of cobalamin to Co is 22 fig. cyanocobalamin to 1 fig- Co. 

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