Gpl20-CCR5 binding

Models also can assist in experimental design and the determination of the limits of experimental systems. For example, it is known that three proteins mediate the interaction of HIV with cells namely, the chemokine receptor CCR5, the cellular protein CD4, and the viral coat protein gpl20. An extremely useful experimental system to study this interaction is one in which radioactive CD4, prebound to soluble gpl20, is allowed to bind to cellular receptor CCR5. This system can be used to screen for... 

Wu L, LaRosa G, Kassam N, et al. Interaction of chemokine receptor CCR5 with its ligands multiple domains for HIV-1 gpl20 binding and a single domain for chemokine binding. J Exp Med 1997 186(8) 1373—1381. 

Understanding the nature of the domains or elements on the chemokine receptor coreceptors is also of significant importance, especially with regard to the development of coreceptor-blocking therapeutics. HIV-1 gpl20 interacts with multiple extracellular domains of the chemokine receptors to mediate binding, and the N-terminus and extracellular loop (ECL) number 2 of CCR5... 

It has been reported that the N-terminal CCR5, the coreceptor for binding of CD4-gpl20 complex, is tyrosine rich and the tyrosines are posttranslationally converted to sulfated tyrosines (54-56). The NMR, X-ray, and docking-based structure of the... 

HIV enters the host cell by fusing the lipid membrane of the virus with the host cell membrane. This fusion is triggered by the interaction of proteins on the surface of the HIV envelope with specific cell surface receptors. One of these is CD4, the main receptor for HIV-1 that binds to gpl20, a surface protein on the virus particle.5 CD4 alone, however, is not sufficient to permit HIV fusion and cell entry-an additional co-receptor from the chemokine family of G-protein coupled receptors (GPCRs) is required. The chemokine receptor CCR5 has been demonstrated to be the major co-receptor for the... 

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