Glycoproteins Glycosylation

C. E. Glycoprotein glycosylation and cancer progression. Biochim Biophys Acta 1999, 1473, 21-34. 

This procedure is not solely specific for carbohydrate side chains of proteins. Unglycosylated proteins may also be stained. To identify glycosylated proteins, the sample should be run in at least two identical lanes cut the gel and stain a lane with the common protein silver stain (Protocols 2.4.2.1 to 2.4.2.4) and the other lane by the described method. Compare pattern and intensity to identify glycoproteins. Glycosylated macromolecules are also stainable with Schiff s reagent (Protocol 2.4.4.1), but with less sensitivity. 

The G-protein coupled receptors are often glycoproteins. Glycosylation sites are located in the extracellular region, e.g. in the form of the consensus sequence Asn-X-Ser/Thr for an N-linked glycosylation. 

Glycoprotein Glycosylation CHO cell Yeast Plant E. colv. Chemical ligation... 

J. W. Dennis, M. Granovsky, and C. F. Warren Glycoprotein glycosylation and cancer progression. Biochimica Biophysica Acta 1473 21-34 (1999). 

Glycoprotein glycosylation sites may be assessed utilizing nano-LC interfaced to a Micromass QTOF Global (Waters, Milford, MA, USA). 

Biosynthesis of Mammalian Glycoprotein Glycosylation Pathways in the Synthesis of the Non-reducing Terminal Sequences , Journal of Biological Chemistry, 254, 12531-41 Beyti, E.D. Porter, J.W. (1976) Biochemistry of Polyisoprenoid Biosynthesis , Annual Reviews of Biochemistry, 45,113-42... 

Keywords 1,2-Diacetals, Glycoproteins, Glycosylation, Protecting groups... 

A universal role of glycoprotein-glycosylation is still obscure [8]. There is, however, evidence that glycan chains of viral glycoproteins may fulfill, at least, two major functions. Inside the cell, i.e. during biosynthesis, they can assist in the folding of... 

Hudgin, R. L., and Schachter, H., 1971, Porcine sugar nucleotide glycoprotein glycosyl-transferases I. Blood serum and liver sialyltransferase. Can. J. Biochem. 49 829-837. 

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