Alteration of glycoprotein glycosylation patterns

O-glycosidie linkages, in whieh the sugar side chain is attached via the hydroxyl group of serine or threonine, or sometimes modified amino aeids sueh as hydroxylysine. 

Carbohydrate side ehains are synthesized by a family of enzymes known as glycosyltrans-ferases. For any glyeoprotein, the exaet eomposition and structure of the carbohydrate side- 

Large deviations from normal batch-to-batch glycosylation pattern is most likely caused by the presence of a glycosidase capable of enzymatic degradation of the carbohydrate side-chains. The likelihood of such eventualities may be minimized by carrying out downstream processing at low temperatures, and as quickly as possible. 

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