Glycoprotein glycosylation site

The G-protein coupled receptors are often glycoproteins. Glycosylation sites are located in the extracellular region, e.g. in the form of the consensus sequence Asn-X-Ser/Thr for an N-linked glycosylation. 

Glycoprotein glycosylation sites may be assessed utilizing nano-LC interfaced to a Micromass QTOF Global (Waters, Milford, MA, USA). 

Kirkpatrick RB, Matico RE, McNulty DE, Strickler JE, Rosenberg M 1995 An abundantly secreted glycoprotein from Drosophila melanogaster is related to mammalian secretory proteins produced in rheumatoid tissues and by activated macrophages. Gene 153 147-154 Leconte I, Carpentier JL, Clauser E 1994 The functions of the human insulin receptor are affected in different ways by mutation of each of the four N-glycosylation sites in the fl subunit. J Biol Chem 269 18062-18071... 

Fig. 8.1 Hypothetical two-dimensional model of human P-glycoprotein. Small circles amino acid residues large circles ATP sites squiggly lines N-linked glycosylation sites (modified from [15]).

M-CSF serves as a growth, differentiation and activation factor for macrophages and their precursor cells. It is also known as CSF-1. This cytokine is produced by various cell types (Table 10.5). The mature form is a glycoprotein containing three potential N-linked glycosylation sites. Three related forms of human M-CSF have been characterized. All are ultimately... 

Human TPO is a 332 amino acid, 60 kDa glycoprotein, containing six potential N-linked glycosylation sites. These are all localized towards the C-terminus of the molecule. The N-terminal half exhibits a high degree of amino acid homology with EPO and represents the biologically active domain of the molecule. 

Examples of Serum and Membrane Glycoproteins Having Multiple O-Glycosylation Sites... 

Fig. 1. Nucleotide sequence of the SFV 26 S RNA (top row), the corresponding amino acid sequence (middle row), and the amino acid sequence of the Sindbis virus structural proteins (bottom row). Nucleotides are numbered from the 5 end of the RNA molecule and all amino adds from the amino terminus of each protein. The amino- and the carboxyl-terminal ends of each protein are indicated hy arrows, glycosylation sites by triangles, and membrane-spanning regions of the viral glycoproteins by underlines for Sindbis virus and overlines for SFV. Amino acids in boxes are negatively charged (Asp and Glu), and those circled are positively charged (Lys and Arg). Some restriction endonuclease cleavage sites are shown on the nucleotide sequence. The alignment of the amino acid...

Imre, T., Schlosser, G., Pocsfalvi, G., Sicihano, R., Mobiar-SzoUosi, E., Kremmer, T., Malorni, A., and Vekey, K., Glycosylation site analysis of human alpha-l-acid glycoprotein (AGP) by capillary liquid chromatography-electrospray mass spectrometry. Journal of Mass Spectrometry 40(11), 1472-1483, 2005. 

The cell-surface EGF receptor also serves as the receptor for the closely related TGF-a growth factor, as discussed later. The receptor gene is located on chromosome 7. The mature product is a 170 kDa glycoprotein, possessing 11 potential glycosylation sites (Figure 7.2). 

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