Glycoproteins 3-glycosidase

Table 47-5. Some glycosidases used to study the structure and function of glycoproteins. ...

The structures of many oligosaccharide chains can be elucidated by gas-liquid chromatography, mass spectrometry, and high-resolution NMR spectrometry. Glycosidases hydrolyze specific linkages in oligosaccharides and are used to explore both the structures and functions of glycoproteins. 

Glycoprotein was digested using the peptide N-Glycosidase F (PNGase F) to release N-linked glycan, then labeled with 8-aminopyrene-l,3,6-trisulfonate (APTS). The detection used LIF with a argon-ion laser. 

Singh, S. Scigelova, M. Crout, D. H. G., Glycosidase-catalysed synthesis of oligosaccharides A two-step synthesis of the core trisaccharide of N-hnked glycoproteins using the beta-N-acetylhexosaminidase and the beta-mannosidase from... 

A. Herscovics, Importance of glycosidases in mammalian glycoprotein biosynthesis, Biochim. Biophys. Acta, 1473 (1999) 96-107. 

Both alkaloids (castanospermine and swansonine) have the ahility to inhihit glycosidase enzymes (GE), the activity of which is necessary in glycoprotein biosynthesis. 

VI (Maroteaux-Lamy syndrome) are very rare, and both are associated with urinary excretion of large amounts of dermatan sulfate, although the diseases differ in their clinical manifestations.389 Other forms of this disease that are, at present, unclassified are known, in which chondroitin 4-sulfate is excreted in excessive amounts in the urine.409,410 It is reasonable to suppose that other glycosidase deficiencies may be found to underlie other genetically acquired diseases that involve glycoproteins. 

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