N-linked glycosylation sites

Fig. 8.1 Hypothetical two-dimensional model of human P-glycoprotein. Small circles amino acid residues large circles ATP sites squiggly lines N-linked glycosylation sites (modified from [15]).

M-CSF serves as a growth, differentiation and activation factor for macrophages and their precursor cells. It is also known as CSF-1. This cytokine is produced by various cell types (Table 10.5). The mature form is a glycoprotein containing three potential N-linked glycosylation sites. Three related forms of human M-CSF have been characterized. All are ultimately... 

Human TPO is a 332 amino acid, 60 kDa glycoprotein, containing six potential N-linked glycosylation sites. These are all localized towards the C-terminus of the molecule. The N-terminal half exhibits a high degree of amino acid homology with EPO and represents the biologically active domain of the molecule. 

Human biotinidase is a monomeric glycoprotein with a molecular mass of 76 kDa when analysed by sodium dodecylsulphate-polyacrylamide gel electrophoresis [7]. The cDNA for human biotinidase was isolated and sequenced in 1994 by Cole and coworkers [6]. The deduced protein contains 502 amino acids, has a molecular mass of 57 kDa and contains 6 potential N-linked glycosylation sites. The biotinidase gene... 

Figure 1 Predicted sequence and membrane topography of the murine delta opioid receptor. The mouse sequence was obtained from Genbank (accession No. L07271). Negatively charged amino acids within TMD 2 and 3 are circled and extracellular N-linked glycosylation sites are indicated ( ). Arrows indicate intron/exon boundaries in the mouse genome. (From Ref. 40.)...

Yu, X. C., Sturrock, E.D., Wu, Z., Bie-mann, K., Ehlers, M.R., Riordan, J.F. Identification of N-linked glycosylation sites in human testis angiotensin-converting enzyme and expression of an active deglycosylated form. J. Biol. Chem. 

The cellular form of the prion protein, called PrP, is synthesized as a 253 amino acid precnrsor protein in hnmans (Figure 29.IB) (Prusiner, 1991). The N-terminal 22 amino acid residues serve as the signal peptide that allows insertion of the nascent PrP peptide into the secretory pathway dnring biosynthesis. The C-terminal 22 amino acid residnes are involved in the covalent addition of the glycosylphosphatidylino-sitol (GPl) moiety to serine at amino acid 231 (i.e., Ser )-The GPI anchor tethers PrP to the extracellnlar side of the plasma membrane. PrP has two N-linked glycosylation sites (Asn and Asn 9 that are the sites of attachment of complex... 

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