Glycopolymers binding

Fig. 15 High-affinity glycopolymer binding to human DC-SIGN and disruption of DC-SIGN interactions with gpl20 [80]...

Polymer adsorption has also been adapted to QCM sensing whereby biofunctional thin films are adsorbed on the crystal surface with non-specific binding controlled by tuning of polymer composition. This approach proved successful as applied to carbohydrate-protein interaction by Matsuura et al. through adsorption of lactose bearing amphiphilic polymers on hydrophobic surfaces which then showed RCA12o and peanut lectin (PNA) affinity [33]. Carbohydrate surfaces prepared by photo insertion into an adsorbed polymer were tested by QCM and showed the predicted affinities [34] while in another example a covalently bound glycopolymer demonstrated Concanavalin A detection ability [35]. 

In a subsequent investigation by the author [ 1 ] multivalent glycopolymers with chain-terminating binding groups, (I), were prepared and used in carbohydrate-mediated biomolecular recognition processes. 

Polypeptide-Based Glycopolymers for the Study of Multivalent Binding Events R. Maheshwari, S. Liu, B.D. Polizzotti, Y. Wang, and K.L. Kiick... 

Polypeptide-Based Glycopolymers for the Study of Multivalent Binding Events... 

Neoglycoproteins, liposomes, and glycopolymers have been successfully used to demonstrate that multivalency does indeed amplify carbohydrate-protein binding interactions by factors as high as thousands. However, by their very nature, these neoglycoconjugates have ill-defined chemical structures. They are heterogeneous in size and carbohydrate contents. Additionally, neoglycoproteins have been shown to... 

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