Glycolipid layer

It has been demonstrated that ILMs are suitable for qualitative and quantitative analyses of low-molecular weight compounds of biological interest, for example, carbohydrates, vitamins and amino acids [38], and glycolipids [40]. ILMs were further used for fhe direct analysis of alkaloids, anesthetics and antibiotics, separated by thin-layer chromatography (TLC) [46]. For this purpose, the ILM was spotted onto the fractions on the TLC-plates and the complete plate was measured in MALDI MS without the need for additional pretreatment of the TLC-samples. The mass deviation inherently caused by the inhomogeneous surface of fhe TLC-plafe was balanced by using the... 

Figure 3.18 Schematic representation of a trilaminar cell membrane which is derived from the apical membrane of the mammary cell and forms the outer layer of the milk fat globule membrane following expression from the mammary cell, but which is more or less extensively lost on ageing. 1, phospholipid/glycolipid 2, protein 3, glycoprotein.

Svennerholm, E. Svennerholm, L. The separation of neutral blood-serum glycolipids by thin-layer chromatography. 

Figure 2. Thin-layer chromatogram of non-acid glycolipids of small intestine of black and white (B) and white (W) rat...

Figure 10. Thin-layer pattern with deduced chemical formulas of non-acid glycolipids of epihtelial cells of the two rat strains (cf. Figure 2)...

Figure 1. Schematic of the effects of brief treatment with mild alkali on the thin-layer chromatographic migrations of three types of glycolipids. 1, 2 control and alkali-treated neutral glycosphingolipid 3, 4 control and alkali-treated galactosyl-alkylacylglycerol 5, 6 control and alkali-treated galactosyldiacylglycerol. OR origin FR solvent front.

The neutral lipid fraction from the DEAE-Sephadex A-50 column was combined with the lower phase obtained after Folch partition of the total lipid extract and the combined lipids dried. To the same flask, 10Q ml of 0.6 M NaOH in methanol was added. The mixture was incubated at 37°C for 5 hours. Five volumes of acetone were then added and stored overnight at 4°C. The precipitate was collected by centrifugation at 4°C and dissolved in C M (4 1, v/v). After application to the column (2.0 x 25 cm), the column was washed with chloroform. Neutral glycolipids were then eluted with tetrahydrofuran H2O (10 1). Fractions containing neutral glycosphingolipids were pooled and their glycolipid content examined by thin-layer chromatography. 

Figure IB. Thin-layer chromatogram of human and chicken neutral glycolipids...

Extensive purification of the glycosphingolipids present in the neutral fraction resulted in the isolation of three distinct glycolipids exhibiting Forssman antigenic activity (Table I, compounds 9,10,11). Thin-layer chromatographs of these glycolipids is illustrated in Fig. 1. Chemical analyses of the purified... 

Figure 1. Thin-layer chromatography of the glycolipids with Forssman activity...

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