Glycine metabolic pool ,turnover

Figure 20.4 The Picou and Taylor-Roberts model for studying whole-body protein turnover. Nitrogen enters the metabolic pool via I and C, and exits via Er and S. [1 N]-glycine is infused continuously, and represents the label for overall nitrogen entry into the pool. (Reproduced with permission from Steffee WP, Goldsmith RS, Pencharz PB, Scrimshaw NS, Young VR. Dietary protein intake and dynamic aspects of whole body nitrogen metabolism in adult humans. Metabolism 25 281-297, 1976.)...

Lapidot A and Nissim L (1980) Regulation of pool sizes and turnover rates of amino acids in humans N glycine and N-alanine single-dose experiments using gas chromatography-mass spectrometry analysis. Metabolism 29, 230-239... 

Urate metabolism was defined by a technique which has been evolved by many investigators over the years. This involved the determination of urate pool and turnover by isotope dilution following the intravenous injection of labelled urate, with the simultaneous measurement of the percent incorporation of glycine labelled with an alternative isotopic label ( C) and determination of its percent incorporation into urinary, and also into produced urate, within a 7 day period. (Methods detailed in "The Effect of Weight Reduction on Urate Metabolism"). 

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