Glycerolphosphate acyltransferases

Mitochondria have an outer membrane that is permeable to most metabohtes, an inner membrane that is selectively permeable, and a matrix within (Figure 12-1). The outer membrane is characterized by the presence of various enzymes, including acyl-CoA synthetase and glycerolphosphate acyltransferase. Adenylyl kinase and creatine kinase are found in the intermembrane space. The phospholipid cardiolipin is concentrated in the inner membrane together with the enzymes of the respiratory chain. 

Figure 10-5. Intrahepatic metabolism of free fatty acids (FFA). CPT I, CPT II, carnitine palmitoyltransferase I, II, respectively LCFA, long-chain fatty acid VLDL, very low-density lipoprotein. 1, Long-chain acyl-CoA synthase 2, acetoacetyl-CoA thiolase 3, hydrox-ymethylglutaryl-CoA synthase 4, hydroxymethylglutaryl-CoA lyase 5, 3-hydroxybutyrate dehydrogenase 6, acetyl-CoA carboxylase 7, fatty acid synthase 8, glycerolphosphate acyltransferase Reprinted with permission from Girard et al. (1992).

Bates, E.J. Saggerson, E.D. (1979) Biochem. J., 182, 751-762. A study of the glycerolphosphate acyltransferase and dihydroxyacetonephosphate acyltransferase activities in rat liver mitochondrial and microsomal fractions. Relative distribution in parenehymal and non-parenehymal cells, effects of N-ethylmaleimide, palmitoyl-CoA concentration, starvation, adrenaleetomy and anti-insulin serum treatment. 

I. Mitchell, J.R.D. Saggerson, E.D. (1994)/nt. J. Biochem., 26, 181-187. A study of glycerolphosphate acyltransferase in rat liver mitochondria-submitochondrial localization and some properties of the solubilized enzyme. 

Bates, E.J. Saggerson, D. (1977) FEES Lett., 84, 229-232. A selective decrease in mitochondrial glycerolphosphate acyltransferase activity in livers from streptozotocin-diabetic rats. 

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