Glycans homogeneous

Alfalfa, clover High yield, useful for animal vaccines, clonal propagation, homogenous N-glycans (alfalfa) Low protein stability in harvested material, presence of oxalic acid... 

Once extracted from the wall, the purification of wall polymers to homogeneity poses further problems. Such purification techniques as combined gel-filtration and ion-exchange chromatography42-55-57-96,97,99,101,102 are being continually improved, but, even so, the heterogeneity and complexity of interconnected glycans constitute an exacting problem. 

This glycan was extracted by a trichloroacetic acid method and purified to homogeneity by alcohol precipitation and bio-gel filtration methods. The complete molecular structure of the glycan has been deduced from data of methylation analysis in combination with enzymic and chemical degradation reactions (25,... 

One method that has been used to acquire carbohydrates is isolation and purification from natural sources such as human or animal tissue, milk, urine, plants, and bacteria (see cross reference Isolation of glycans). Access to homogeneous carbohydrate stmctures can be challenging due to the difficulties in separation of complex mixtures, identification of carbohy-drate(s) contained within each fraction, and preparation of sufficient quantities from the limited amounts present in a particular sample. Alternatively, mixtures of unknown composition can be used to survey a broad repertoire of the glycome. On identification of a mixture containing one or more members with interesting receptor-binding properties, the mixture can then be deconvoluted by further fractionation and separation by routine analytical techniques (13). 

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