Glutathione-S-transferase , fusion

Shimada K, Nagano M, Kawai M, Koga H. (2005) Influences of amino acid features of glutathione S-transferase fusion proteins on their solubility. Proteomies 5, 3859-63. 

Murray AM, Kelly CD, Nussey SS, Johnstone AP. (1998) Production of glutathione-coated microtitre plates for capturing recombinant glutathione S-transferase fusion proteins as antigens in immunoassays. J Immunol Methods 218, 133-9. 

Waterboer, T., et al. (2005) Multiplex hiunan papillomavirus serology based on in situ-purified glutathione s-transferase fusion proteins. Clin Chem. 51, 1845-53. 

Weiss, S., Famulok, M., Edenhofer, F, Wang, Y.H., Jones, I.M., Groschup, M., and Win-nacker, E.L. (1995). Overexpression of active Syrian golden hamster prion protein PrPc as a glutathione S-transferase fusion in heterologous systems. J. Virol. 69, 4776-4783. 

Additional information <27, 29> (<27>, a glutathione S-transferase fusion protein of Clk3 catalyzes autophosphorylation of the kinase but not phosphorylation of the exogenous substrates histone or casein [45] <29>, activity is dependent on tyrosine residues between subdomains VII and VIII [47]) [45, 47]... 

Haun, R. S., and Moss, J. (1992). Ligation-independent cloning of glutathione S-transferase fusion genes for expression in Escherichia coli. Gene 112, 37-43. 

Redfern, C P. F and Wilson, K E (1993) Ligand-bmding properties of human cellular retinoic acid-bindmg protein-II expressed in Escherichia-coli as a glutathione-s-transferase fusion protein. FEBS Letts 321, 163-168... 

Figure 1 Western blots of total cellular proteins of BL21(DE3) containing KAS 1 expression constructs. Cells were grown in LB at 37 C to an OD6oo of 0.6, and then induced for 3 hrs by addition of 0.4 mM IPTG. Aliquots were tak before (u) and after induction (i) (50p.l and 30)xl of culture respectively). Cells were harvested and proteins separated on a 12% SDS page gel. After transfer to a nitro-cellulose membrane, western blots were prepared using primary antibodies raised in rabbits. The antigens were a recombinant glutathione-S-transferase fusion with KAS 12 and purified FAB B.

Ohsako, S., Janulis, L., Hayashi, Y., and Bunick, D. Characterization of Domains in Mice of Calnexin-t, a Putative Molecular Chaperone Required in Sperm Fertility, with Use of Glutathione S-transferase-fusion Proteins Biol. Reprod. 1998 59, 1214-23. 

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