Crosslinking agents glutaraldehyde

Polyethyleneimine (PEI) (Scheme 3) was immobilized onto cellulose fibers by a crosslinking agent glutaraldehyde (21). 10-60 wt% of PEI was attached by varying the mass ratio of PEI to cellulose. The cellulose-PEI fibers with 20-60 wt% of PEI adsorbed up to 10 mg endotoxin per g of modified fibers and could remove 90-100% endotoxin from 500 ng/ml endotoxin solution at pH 7.0 and ionic strengths lower than 0.4. In addition, the cellulose-PEI fibers with 20 wt% PEI showed selective removal activity toward endotoxins from various protein solutions at pH 7 and 0.05 ionic strength. This selective adsorption was due to simultaneous effects of cationic properties of amino groups and... 

Glutaraldehyde was one of the first and still is one of the most commonly used crosslinking agents available for creating antibody-enzyme conjugates. The crosslinking process using... 

In addition to ABS, membranes using a polymer blend of chitosan and ABS with glutaraldehyde as a crosslinking agent have been used as coating materials for PES ultrafiltration membranes (90). 

We have synthesized aldehyde-functionalized silanes as a new class of spacer molecules for silica surfaces. The aldehyde group can be used to attach biomolecules directly to the surface, with no bifunctional crosslinking species required (Fig. IB) [3]. As many immobilization problems are closely related to the use of the crosslinking agent (e.g., glutaraldehyde tends to form undesirable polymers) [4], the development of a one-step immobilization method is a significant improvement. 

It is assumed that the partially denaturated protein binds the inhibitor whereby a new active center is formed which is similar to that of the model enzyme. In one of several examples transformation of a trypsin to a chymo-trypsin-active protein is described with indole as inhibitor and glutaraldehyde as crosslinking agent. The new product showed an increase of chymotrypsin activity of A00% and a decrease of trypsin activity of 1AZ. 

Chitin and chitosan have been extensively employed for the immobilization of enzymes and cells applied in food technology and in biosensors [208], Enzymes immobilized in these polymers possess an increased stability and are more temperature resistant. The immobilized enzyme ean usually be recovered after reaction and reused. Immobilization is often achieved using glutaraldehyde as the crosslinking agent, although the enzymes have also... 

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