Glucosidase II

A. Hentges and E. Bause, Affinity purification and characterization of glucosidase II from pig liver, Biol. Chem., 378 (1997) 1031-1038. 

Fig. 2. Oligosaccharide processing reactions occurring in the ERin mammalian cells. N, M, G, Dol, Prot, OST, GI, GII, MI, Mil, and GT stand for GlcNAc, Man, Glc, protein, dolichol, glucosidase I, glucosidase II, mannosidase I, mannosidase II, and UDP-GIc glycoprotein glucosyltransferase, respectively.

Trombetta, E. S., Simons, J. F., and Helenius, A. (1996). Endoplasmic reticulum glucosidase II is composed of a catalytic subunit, conserved from yeast to mammals, and a tightly bound noncatalytic HDEL-containing subunit. J. Biol. Chem. 271, 27509-27516. 

Reitman, M. L., Trowbridge, L. S., and Kornfeld, S. (1982). A lectin-resistant mouse lymphoma cell line is deficient in glucosidase II, a glycoprotein processing enzyme. J. Biol. Chem. 257, 10357-10363. 

Reagents i, /1-Glucosidase ii, Hj-Pd/C-MeOH-AcOH iii, NaBH4-MeOH iv. PbtOAcli V, CH Nj vi, Hj-catalyst. 

Reagents i, -glucosidase ii, prolonged methylation treatment -CHjN iii, H -Pd/C-MeOH-AcOH iv, brief CHjNj... 

Deoxynojirimycin Bacillus Inhibits glucosidase 1 and in high doses also glucosidase II 150 to 800 gg/ ml ... 

FIGURE 14.8 Electropherogram of an a-glucosidase II single-cell (Cell 7) reaction aliquot taken after incubation (a). Percent conversion of the dissaccharide (DG-TMR) substrate by a-glucosidase II in single cells and control assays (b). Percent conversion was calculated by comparing the DG-TMR peak to that of the monosaccharide product MG-TMR. (From Shoemaker, GK. et al.. Anal. Cheni., 77, 3132, 2005. With permission.)... 

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