Glucose 6-phosphate, sources, preparation

Phosphorolysis of ribonucleic acid with polynucleotide phosphorylase gives a mixture of the diphosphates of the four common nucleosides, which are transformed into triphosphates with enolpyruvate phosphate and pyruvate kinase. This mixture may be used as such as a source of uridine triphosphate in the preparation of the nucleotide-sugar uridine 5 -(a-D-glucopy-ranosyl diphosphate) ( uridine-diphosphate-glucose, UDP-Glc), or as a... 

The transfer of glucose from dolichyl glucosyl phosphate to oligosaccharides attached to protein has since been found in microsomal preparations from several sources and seems to be a common phenomenon. It has also been shown with thyroid slices by Spiro et al (1976a, b, c) so... 

Preparation. According to Plattner and Clauson-Kass, lycomarasmin is isolated as follows Fusarium lycopersici is cultured in a glucose-mineral medium whose nitrogen source is ammonium nitrate. After 2 to 4 months, the maximum concentration of lycomarasmin in the medium (as measured by blighting power for the tomato) is attained. The mycelium is removed by filtration, sulfate, and phosphate are precipitated by baryta, and ammonia is removed by vacuum distillation. The active substance remains in solution from which, on acidification with HCl, and addition of ethanol, lycomarasmin crystallizes in fine needles. 

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