Glucose oxidase characterization

Dalmasso, P., Pedano, M.L., and Rivas, GA. (2013) Supramolecular architecture based on the self-assembling of multiwall carbon nanotubes dispersed in polyhistidine and glucose oxidase characterization and analytical applications for glucose biosensing. Biosens. Bioelectron., 39, 76—81. 

J.P. Lowry and R.D. O Neill, Partial characterization in vitro of glucose oxidase modified poly(phenylenediamine)-coated electrodes for neurochemical analysis in vivo. Electroanalysis 6, 369— 379 (1994). 

The blend of PVA with PEG- modified glucose oxidase could be used as glucose sensor characterized by the linearity of calibration curve in the range of concentration by 5 x 10"5 - 5 x 10"3 mol glucose L 1 [194],... 

The nitrous acid deamination of 2-amino-2-deoxy-D-mannose (8), in the favored conformation, the amino group ofwhich is axially attached, leads, in contrast, uniquely to D-glucose,15 characterized, after oxidation by nitric acid, as D-glucaric acid (9). This result has also been verified by direct crystallization of the D-glucose and by assay with D-glucose oxidase.44"... 

S. Campuzano, R. Galvez, M. Pedrero, F.J. Manuel de Villena and J.M. Pingarron, Preparation, characterization and application of alkanethiol self-assembled monolayers modified with tetrathiafulvalene and glucose oxidase at a gold disk electrode, J. Electroanal. Chem., 526(1-2) (2002) 92-100. 

Electrochemical Characterization of Ferrocene Derivatives in a Perfluoropolymer Glucose Oxidase Electrode... 

Preparation and Characterization of Active Glucose Oxidase Immobilized to a Plasma-Polymerized Film... 

This study on the immobilization of glucose oxidase and the characterization of its activity has demonstrated that a bioactive interface material may be prepared from derivatized plasma polymerized films. UV/Visible spectrophotometric analysis indicated that washed GOx-PPNVP/PEUU (2.4 cm2) had activity approximately equivalent to that of 13.4 nM GOx in 50 mM sodium acetate with a specific activity of 32.0 U/mg at pH 5.1 and room temperature. A sandwich-type thin-layer electrochemical cell was also used to qualitatively demonstrate the activity of 13.4 nM glucose oxidase under the same conditions. A quantitatively low specific activity value of 4.34 U/mg was obtained for the same enzyme solution by monitoring the hydrogen peroxide oxidation current using cyclic voltammetry. Incorporation of GOx-PPNVP/PEUU into the thin-layer allowed for the detection of immobilized enzyme activity in 0.2 M sodium phosphate (pH 5.2) at room temperature. 

The available knowledge about the structural and mechanistic properties of flavoenzymes is extremely valuable for the discovery and characterization of new flavoenzymes and for the development of practical applications. Several fiavoenzyme-inspired applications already exist but many more can be foreseen. Glucose oxidase (EC 1.1.3.4) (27) and cholesterol oxidase (EC 1.1.3.6) (28) are widely applied in diagnostics. Many fiavopro-tein oxidases and monooxygenases serve as biocatalysts for the... 

Franchina J. G., Lackowski W. M., Dermody D. L., Crooks R. M., Bergbreiter D. E., Sirkar K., Russell R. J., and Rishko M. V, Electrostatic immobilization of glucose oxidase in a weak acid, polyelectrolyte hyperbranched ultrathin film on gold Fabrication, characterization, and enzymatic activity, A a/. Chem., 71(15), 3133-3139,1999. 

M. Zhao, X. Wu, and C. Cai, Polyaniline nanofibers Synthesis, characterization, and application to direct electron transfer of glucose oxidase, J. Phys. Chem. C, 113, 4987-4996 (2009). 

Demura, M. Asakura, T. Porous membrane of Bombyx mori silk fibroins Structure characterization, physical properties and application to glucose oxidase immobilization. J. Membr. Sci. 59 39-52 (1991). 

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