Glucose invertase electrode

Scheller F, Karsten C. A combination of invertase reactor and glucose oxidase electrode for the successive determination of glucose and sucrose. Anal Chim Acta 1983 155 29-36. 

Matsumoto et al (41) prepared a multi-enzyme electrode using glucose oxidase, invertase, mutarotase, fructose-5-dehydrogenase, and catalase to simultaneously detect glucose, fructose, and sucrose in fruit juices and soft drinks. Detection of multi-components by enzyme sensors was also reported in analysis of sucrose and glucose in honey (42) and drinks (43), and L-malate and L-lactate in wines (44). 

Jawaheer et al. [12] Fruits (maturity and quality) Glucose oxidase, mutarotase, invertase/ pectin matrix and covered with a cellulose acetate (CA) membrane Screen-printed rhodinised carbon electrodes/ +350mV vs. Ag/AgCl ... 

In invertase-GOD electrodes glucose is formed in the following sequence of reactions ... 

Fig. 78. Determination of sucrose with immobilized invertase and a GOD electrode, (a) Measuring cell, (b) Measuring curves of successive assay of glucose and sucrose. 1 current indication (/), 2 kinetic indication (d//df).

These examples indicate the in situ applicability of enzyme electrodes however, numerous problems have still to be solved. At present, coupling of enzyme sensors for fermentation control in a bypass arrangement appears to be more favorable [412]. Following this concept, an invertase thermistor incorporating a sterilizable filter unit has been developed [413] for the monitoring of alcoholic fermentation by immobilized yeast cells. Another thermistor has been successfully used for on-line glucose measurement under real cultivation conditions of Cephalosporium acremonium [414]. Similar calorimetric devices are suitable for other fermentation processes and in environmental analysis. 

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