Gluconate, determination

J. Yoshimura and M. Matsuzawa, Stereoselective synthesis of methyl 4-C-acetyl-6-deoxy-2,3-O-methylene-D-galactonate and -D-gluconate. Determination of the D-galacto configuration of methyl eurekanate by synthesis, Carbohydr. Res. S5 C1 (1980). 

In this experiment phosphate is determined by singlecolumn, or nonsuppressed, ion chromatography using an anionic column and a conductivity detector. The mobile phase is a mixture of n-butanol, acetonitrile, and water (containing sodium gluconate, boric acid, and sodium tetraborate). 

In presence of the enzyme glucose oxidase, an aqueous solution of glucose undergoes oxidation to gluconic acid with formation of hydrogen peroxide which can be determined by anodic oxidation at a fixed potential. 

A second principle used widely for glucose analysis, is that of the oxidation of glucose enzymatically, mediated by the action of glucose oxidase with the formation of gluconic acid and hydrogen peroxide (22). In this procedure it is the hydrogen peroxide which is usually assayed for determination of glucose. This method suffers from the action of inhibitors which occur, particularly with patients in a diabetic coma and these need to be removed. 

Mato, I., Huidobro, J. F., Sanchez, M. P., Muniategui, S., Fernandez-Muino, M. A., and Sancho, M. T. (1997). Enzymatic determination of total o-gluconic acid in honey. /. Agric. Food Chem. 45, 3550-3553. 

As a corollary to this work, oxidation with periodate constitutes the basis of analytical procedures for the determination of 6-deoxyhexoses ( methylpentoses ),88 calcium gluconate89 and gluconic acid,90 hexitols,91 94... 

The process in Example 12-7 was conducted by initially adding 0.35 mol glucose to the 100-L reactor, and replenishing the reaction with 0.28 mol flesh glucose every 144 h for 30 days. Determine the gluconic acid and glucose concentration-time profiles in the reactor over this period... 

Mansour, M., Thaller, S., andKorte, F. Action of sunlight on parathion. Bull. Environ. Contam. Toxicol, 30(3) 358-364,1983. Manzurola, E. and Apelblat, A. Solubilities of L-glutamic acid, 3-nitrobenzoic acid, p-toluic acid, calcium-L-lactate, calcium gluconate, magnesium-DL-aspartate, and magnesium-L-lactate in water, J. Chem. Thermodyn., 34 (7) 1127-1136, 2002. Maraqa, M.A., Zhao, X., Wallace, R.B., and Voice, T.C. Retardation coefficients of nonionic organic compounds determined by batch and column techniques. Soil Sci. Soc. Am. J., 62(1) 142-152, 1998. 

After appropriate dilutions, samples collected for concentration determinations were analysed using the sodium gluconate - potassium fluoride method on a dosimate-titrator (Kirke, E.A., Alcoa of Australia, Kwinana, personal communication, 1986). Total alkaline, totd caustic and alumina concentrations were determined using this method. 

Test dose Administer a single 200 mg tablet of quinidine sulfate or 200 mg IM quinidine gluconate to determine whether the patient has an idiosyncratic reaction. Adjust the dosage to maintain plasma concentration between 2 to 6 mcg/mL. 

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